Catalase−polyethylene glycol
SIGMA/C4963 - lyophilized powder, ~40,000 units/mg protein
Synonym: PEG-Catalase
MDL Number: MFCD00676653
Product Type: Chemical
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| composition | Protein, ~50% E405 |
| extent of labeling | ~40 mol PEG per mol protein |
| form | lyophilized powder |
| matrix attachment | secondary amine linkage. |
| mol wt | PEG 5,000 |
| Quality Level | 200  |
| specific activity | ~40,000 units/mg protein |
| storage temp. | −20°C |
| Application: | Pulmonary artery endothelial cells treated with PEG-catalase showed effective inhibition of 20- HETE (hydroxyeicosatetraenoic acid)-induced increase in fluorescence. However, the experiment excludes potential nonspecific fluorescence of DCF (dichlorofluorescein). This experiment studied the effect of 20-HETE on superoxide production and NADPH oxidase activation. |
| Biochem/physiol Actions: | Catalase from bovine liver catalyzes the decomposition of H2O2 into water and oxygen. It is a tetramer consisting of four equal subunits with a molecular weight of 60 kDa each. Each subunit contains iron bound to a protoheme IX group. The enzyme also strongly binds NADP, which is in close proximity to the heme group. Catalase activity is constant over the pH range of 4.0-8.5. The pI is found to be 5.4. The enzyme activity is inhibited by 3-amino-1-H-1,2,4 triazole, cyanide, azide, hydroxylamine, cyanogen bromide, 2-mercaptoethanol, dithiothreitol, dianisidine, and nitrate. Incubation of catalase with ascorbate or ascorbate/Cu2+ results in degradation of the catalase molecule. It does not require any activators. |
| Other Notes: | Catalase from bovine liver coupled to methoxy-polyethylene glycol. |
| Packaging: | Package size based on protein content |
| Physical form: | Contains PEG plus 5% citrate buffer salts |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ~40,000 units/mg protein |
| Storage Temp. | −20°C |
| UNSPSC | 12352204 |