Cytochrome P450 human
SIGMA/C4982 - 3A4 isozyme microsomes, with P450 reductase and cytochrome b5, recombinant, expressed in baculovirus infected insect cells (BTI-TN-5B1-4)
Synonym: Cytochrome P450 enzyme; Human cytochrome P450; P450 enzyme
MDL Number: MFCD03456520
Product Type: Chemical
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| application(s) | cell analysis |
| biological source | human |
| concentration | ≥2 mg/mL (Bichinchonic acid method) |
| form | solution |
| mol wt | 45-60 kDa |
| packaging | vial of ≥25 units |
| vial of ~0.5 nmol | |
| Quality Level | 200  |
| recombinant | expressed in baculovirus infected insect cells (BTI-TN-5B1-4) |
| shipped in | dry ice |
| solubility | water: soluble |
| storage temp. | −70°C |
| suitability | suitable for molecular biology |
| technique(s) | co-immunoprecipitation (co-IP): suitable |
| UniProt accession no. | P08684  |
| Application: | Cytochrome P450 human has been used in in vitro binding assays to understand protein dimerization via haem–haem stacking and its significance in cancer. It has also been used to study in vitro binding between PGRMC1 and CYP3A4. |
| Biochem/physiol Actions: | Cytochrome P450 (CYP) enzymes are associated with many reactions including O-dealkylation, epoxidation, S-oxidation, and hydroxylation. |
| Biochem/physiol Actions: | Cytochrome P450 is a heterogeneous family of isozymes whose primary function is to oxidize small molecules, both as a function of intermediary metabolism (e.g., fatty acids) and to detoxify exogenous compounds (drugs or toxins). Some isoforms have narrow substrate specificity, while others are promiscuous. The CYP1A1 isoform catalyzes 7-deethylation of ethoxyresorufin. Cytochrome P450 (CYP) plays an important role in detoxifying xenobiotics, cellular metabolism and homeostasis. One of the main mechanisms of drug-drug interactions is the induction or inhibition of these enzymes. CYP enzymes are transcriptionally activated by a variety of xenobiotics and by endogenous substrates via receptor-dependent pathways. Inhibition of these enzymes is a major factor in metabolism-based drug-drug interactions, and many chemotherapeutic medications can cause drug interactions by either inhibiting or inducing the cytochrome p450 enzyme system. |
| General description: | Research area: IMMUNO AND CKS Cytochrome P450 (CYP450) is a membrane-bound hemoprotein that constitutes a vast superfamily of heme-thiolate proteins engaged in the metabolism of exogenous and endogenous compounds. These CYP450 enzymes feature an active heme iron center that is bound to a protein molecule via a highly conserved cysteine thiolate ligand. |
| Other Notes: | Microsomes containing recombinant human CYP3A4 and recombinant rabbit NADPH-P450 reductase |
| Other Notes: | One unit will convert 1 nanomole of testosterone to 6β-hydroxytestosterone per minute at pH 7.4 at 37 °C. |
| Physical form: | Solution in 100 mM potassium phosphate buffer, pH 7.4. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −70°C |
| Enzyme Commission (EC) Number | 1.6.2.4 ( BRENDA | IUBMB ) |
| UNSPSC | 12161501 |