Monoclonal Anti-Cytokeratin Peptide 4 antibody produced in mouse
SIGMA/C5176 - clone 6B10, ascites fluid
Synonym: Anti-CK-4; Anti-CK4; Anti-CYK4; Anti-K4; Anti-WSN1
MDL Number: MFCD00211841
Product Type: Chemical
Immunohistochemistry Staining of formalin-fixed, paraffin-embedded human tongue sections with 1:300 Monoclonal Anti-Cytokeratin Peptide 4, Clone: 6B10 (Cat. No. C5176) using Goat Anti-Mouse IgG, FITC-conjugate (Cat. No. F5262).
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 6B10, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| isotype | IgG1 |
| mol wt | antigen 59 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | rabbit, feline, hamster, goat, guinea pig, human, canine, sheep |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable |
| immunohistochemistry (frozen sections): suitable | |
| indirect immunofluorescence: 1:300 using formalin-fixed, paraffin-embedded sections of human or animal tissue | |
| microarray: suitable | |
| western blot: suitable | |
| UniProt accession no. | P19013  |
| Application: | Monoclonal Anti-Cytokeratin Peptide 4 antibody is suitable for use in a broad range of immunoassays such as immunohistochemistry (frozen or formalin-fixed, paraffin-embedded sections), immunoblot, dot blot, and indirect immunofluorescence (1:300 using formalin-fixed, paraffin-embedded sections of human or animal tissue). The antibody may also be used for western blot and microarray. |
| Biochem/physiol Actions: | The antibody reacts with non-cornifying squamous epithelium (e.g., exocervix, esophagus and cornea). Ciliated pseudostratified epithelium of bronchi is weakly stained. Ducts of sweat glands and prostate are focally stained. The antibody may react with some endocervical columnar cells. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Cytokeratins are intermediary filaments that are expressed during epithelial development. Cytokeratin-4 is largely found in non-epidermal, stratified epithelial tissues such as larynx, pharynx, vagina, exocervix and epiglottis among several others . Cytokeratin-4 has been implicated in Streptococcus agalactiae infection and genetic mutations in Cytokeratin-4 have been linked to white sponge naevus . Monoclonal Anti-Cytokeratin Peptide 4 antibody is specific for cytokeratin 4 in goats, hamsters, guinea pigs, cats, sheep, humans, rabbits, and dogs. The antibody weakly stains ciliated pseudostratified epithelium of bronchi. Ducts of skin sweat glands and prostate are focally stained by the antibody. Some endocervical columnar cells may also react with the antibody. This antibody is reactive with methanol or acetone fixed frozen sections, and with protease-digested formalin fixed paraffin embedded human tissues. Embedded methacarn fixed material can also be used for cytokeratin 4 demonstration. |
| Immunogen: | cytokeratin from human esophagus. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |