Carboxypeptidase B from human pancreas
SIGMA/C5233 - 50-55 units/mg protein carboxypeptidase B
Synonym: Peptidyl-L-Lysine[L-arginine] hydrolase
CAS Number: 9025-24-5
MDL Number: MFCD00081476
Product Type: Chemical
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| biological source | human pancreas |
| form | solution |
| impurities | ≤0.2% chymotrypsin |
| ≤0.2% trypsin | |
| ≤1 unit/mg protein carboxypeptidase A | |
| InChI | 1S/C31H38N4O7S/c1-20(32-2 |
| InChI key | TWURVFFNODFJBJ-UHFFFAOYSA |
| Quality Level | 200  |
| shipped in | dry ice |
| SMILES string | S(Cc3ccccc3)C(=O)C(NC(=O) |
| specific activity | 50-55 units/mg protein carboxypeptidase B |
| storage temp. | −20°C |
| UniProt accession no. | P15086  |
| Application: | Carboxypeptidase B from Sigma has been used as a reference for assaying carboxypeptidase activity in lysed pituitary granules derived from the anterior and intermediate lobes of rat. The enzyme has also been used to digest plasma samples by removing C-terminal basic amino acids, to get a distinct band for each allotype during C4 electrophoresis. |
| Biochem/physiol Actions: | Carboxypeptidase B (or peptidyl-L-lysine (-L-arginine) hydrolase) catalyzes the hydrolysis of the basic amino acids, lysine, arginine, and ornithine from the C-terminal position of polypeptides. It has been shown to be a single polypeptide of 34,000 Da. Trypsin is capable of converting native enzyme to the active enzyme, carboxypeptidase B II in vitro. The optimum pH is found to be 9.0. The enzyme may be used for sequence analysis by successive cleavage of C-terminal basic amino acids. It can also be used as a serum marker for the diagnosis of acute pancreatitis. |
| Biochem/physiol Actions: | Mutations in the carboxypeptidase B (CPB1) gene is implicated with increased susceptibility to pancreatic cancer development and progression. Elevated levels of CPB1 is associated with low grade breast tumors and lymph node positive grade 1 tumors. |
| General description: | Carboxypeptidase B is mapped to human chromosome 3q24. Carboxypeptidase B belongs to A/B subfamily of carboxypeptidases. |
| Other Notes: | One unit will hydrolyze 1 μmole of hippuryl- |
| Physical form: | Solution in 0.05 M NaOAc pH 5.0 + 1.0 M NaCl + 0.01% NaN3 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: 50-55 units/mg protein carboxypeptidase B |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 3.4.17.2 ( BRENDA | IUBMB ) |
| UNSPSC | 12352204 |