CelLytic™ IB Inclusion Body Solubilization Reagent
SIGMA/C5236
Product Type: Chemical
Figure 1. Solubilization of Streptavidin Inclusion Bodies.
Samples of solubilized inclusion body protein were assayed using BCA Reagent (Cat. No. BCA-1). 50 μl of each sample were incubated with BCA reagent at 60 °C for 15 min. The samples were then cooled to room temperature and assayed at 562 nm. The data above has been standardized to the protein recovery of CelLytic IB.
Samples of solubilized inclusion body protein were assayed using BCA Reagent (Cat. No. BCA-1). 50 μl of each sample were incubated with BCA reagent at 60 °C for 15 min. The samples were then cooled to room temperature and assayed at 562 nm. The data above has been standardized to the protein recovery of CelLytic IB.
| form | liquid |
| Quality Level | 200  |
| storage temp. | room temp |
| Application: | CelLytic™ IB was designed to solubilize protein aggregates called inclusion bodies, which are formed in bacteria when recombinant proteins are overexpressed. CelLytic™ IB was formulated to solubilize the protein of interest for immediate analysis of protein content or refolding procedures. |
| Legal Information: | CelLytic is a trademark of Sigma-Aldrich Co. LLC |
| Packaging: | 25 mL in poly bottle |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H302 - H315 - H319 |
| Precautionary statements | P264 - P270 - P280 - P301 + P312 - P302 + P352 - P305 + P351 + P338 |
| Hazard Codes | Xn |
| Risk Statements | 22-36/38 |
| Safety Statements | 26 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | room temp |
| UNSPSC | 41116134 |