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Concanavalin A from Canavalia ensiformis (Jack bean)

SIGMA/C5275 - Type IV-S, lyophilized powder, aseptically processed, BioReagent, suitable for cell culture

Synonym: Con A

CAS Number: 11028-71-0
EC Number: 234-258-2
MDL Number: MFCD00071069
Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-C5275-5MG 5 mg
 $76.70
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form lyophilized powder
impurities salt (Contains buffer salts and NaCl)
product line BioReagent
Quality Level 200 
solubility H2O: soluble, slightly hazy
  PBS: 5 mg/mL, slightly hazy
sterility aseptically processed
storage temp. −20°C
technique(s) cell culture | mammalian: suitable
type Type IV-S
Analysis Note: A lymphocyte transformation assay is used to determine suitability for cell culture applications. The mitogenic activity of this product is assessed by bromodeoxyuridine incorporation and is found to have peak incorporation at ≤75 ug lectin/ml of medium.
Where reported, agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, containing Ca2+ and Mn2+ of a 1 mg per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes after 1 hr incubation at 25 °C.
Application: Concanavalin A from Canavalia ensiformis (Jack bean) has been used:
• in in vitro cell proliferation assay and cell cycle assessment
• in lymphocyte proliferation
• in splenocyte culturing
Biochem/physiol Actions: Con A is not blood group specific but has an affinity for terminal α-D-mannosyl and α-D-glucosyl residues. Ca2+ and Mn2+ ions are required for activity. Con A dissociates into dimers at pH 5.6 or below. Between pH 5.8 and pH 7.0, Con A exists as a tetramer; above pH 7.0 higher aggregates are formed. Con A exhibits mitogenic activity which is dependent on its degree of aggregation. Succinylation results in an active dimeric form which remains a dimer above pH 5.6.
Biochem/physiol Actions: Concanavalin A is known to stimulate acute hepatic inflammation and acts as a T‐cell mitogen. It has inhibitory effect on hepatocellular carcinoma by inducing autophagy.
General description: Concanavalin A is a protein containing only β-sheets in its structure, and belongs to the legume lectins family.
Storage Temp. −20°C
UNSPSC 23151817

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