Anti-Corticotropin Releasing Factor antibody produced in rabbit
SIGMA/C5348 - IgG fraction of antiserum, buffered aqueous solution
Synonym: CRF Antibody - Anti-Corticotropin Releasing Factor antibody produced in rabbit; Crf Antibody; Anti-CRF
MDL Number: MFCD02096145
Product Type: Chemical
Immunohistochemistry Formalin fixed, paraffin-embedded, microwave-treated sections of Chicken intestine tissue were stained with 1:100 Anti-Corticotropin Releasing Factor antibody produced in Rabbit (Cat. No. C5348), followed by 10 μg/mL Anti-Rabbit IgG (whole molecule)-Biotin antibody produced in Goat (Cat. No. B8895) and by 20 μg/mL ExtrAvidin®-Peroxidase (Cat. No. E2886).
| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | frog, canine, sheep, rat, human, mouse, pig, bovine |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100 using microwave-treated sections of human placental tissue |
| microarray: suitable | |
| radioimmunoassay: 1:10,000 using 2-125I-His12-CRF, human | |
| UniProt accession no. | P06850  |
| Application: | Anti-Corticotropin Releasing Factor antibody produced in rabbit has been used in immunohistochemistry (1:2000) and radioimmunoassay. |
| Biochem/physiol Actions: | Corticotropin Releasing Factor (CRF) is critical in the regulation of the hypothalamic-pituitary-ad |
| Biochem/physiol Actions: | The antibody does not cross-react with human or rat CRF (6-33), sheep CRF, rat urocortin, PACAP38, human ACTH, human ACTH (18-39) and sauvagine. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Corticotropin Releasing Factor (CRF), a 41 amino acid neuropeptide, belongs to a family of structurally related peptides including urocortin, sauvagine and urotensin I. CRF is derived from a larger 196 amino acids precursor protein (preproCRF). CRF is widely distributed in the brain and peripheral nervous system, with the highest levels in the hypothalamic paraventricular nucleus (PVN). |
| Immunogen: | synthetic peptide corresponding to the C-terminal of human CRF (amino acids 25-41 with N-terminally added lysine) conjugated to KLH. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Preparation Note: | For continuous use, store at 2-8°C for up to one month. For extended storage freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |