Monoclonal Anti-Actin–Cy3 antibody produced in mouse
SIGMA/C5838 - clone AC-40, purified immunoglobulin, buffered aqueous solution
Synonym: Monoclonal Anti-Actin
Product Type: Chemical
Immunofluorescence Chicken fibroblasts cells were fixed and permeabilized with methanol followed by acetone. Fixed cells were stained and developed with 1:100 Monoclonal Anti-Actin-Cy3 antibody produced in Mouse, Clone: AC-40 (Cat. No. C5838).
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | AC-40, monoclonal |
| conjugate | CY3 conjugate |
| form | buffered aqueous solution |
| isotype | IgG2a |
| mol wt | antigen 42 kDa |
| Quality Level | 200  |
| shipped in | wet ice |
| species reactivity | human, Xenopus, hamster, mouse, guinea pig, rat, rabbit, snail, sheep, canine, carp, chicken, pig, bovine, viper, goat |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | direct immunofluorescence: 1:75 using cultured human or chicken fibroblasts |
| Application: | Monoclonal anti-actin antibody was used for western blot analysis of Cos-7 cell lysates to ensure equal protein loading. |
| Application: | Monoclonal Anti-Actin-Cy3 antibody has been used in immunofluorescent staining. |
| Application: | Western blot analysis of MDCK cell lysates were performed using monoclonal anti-actin antibody as a primary antibody. |
| Biochem/physiol Actions: | Actin and myosin are constituents of many cell types and are involved in a myriad of cellular processes including locomotion, secretion, cytoplasmic streaming, phagocytosis and cytokinesis. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-Actin (mouse IgG2a isotype) is derived from the AC-40 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Actin has six isoforms. Four of them represent the differentiation markers of muscle tissues and two are found practically in all cells. There are three α-actins (α-skeletal, α-cardiac and α-smooth muscle), one β-actin (β-nonmuscle) and two γ-actins (γ-smooth muscle and γ-nonmuscle). |
| General description: | The antibody recognizes an epitope located on the C-terminal end of actin conserved in all actin isoforms. It specifically labels actin in a wide variety of tissues and species. The epitope recognized by the antibody is resistant to formalin-fixation and paraffin-embedding. Zinc-formalin, B5, ethanol, methacarn, Brunnel′s or Bouin′s solutions may also be used as fixatives. |
| Immunogen: | synthetic actin C-terminal peptide, attached to a Multiple Antigen Peptide (MAP) backbone |
| Legal Information: | Distributed under license from Amersham Biosciences Limited. |
| Other Notes: | To view an Actin antibody selection guide, please visit www.sigmaaldrich.com/acti. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |