Choline Oxidase from Alcaligenes sp.
SIGMA/C5896 - lyophilized powder, ≥10 units/mg solid
Synonym: Choline: oxygen 1-oxidoreductase
CAS Number: 9028-67-5
EC Number: 232-840-0
MDL Number: MFCD00130787
Product Type: Chemical
Figure 1. Stability (powder form); kept under dry conditions
Figure 2. Stability (liquid form at 37 °C); buffer composition: 0.1 M K-phosphate buffer, pH 7.5
Figure 3. pH Activity; 37 °C, in 50 mM K-phosphate buffer
Figure 4. Temperature activity; in 50 mM K-phosphate buffer, pH 7.5
Figure 5. pH Stability; 30 °C, 2-hr treatment with 50 mM buffer solution: pH 6.0–9.0, K-phosphate; pH 9.0–10.0, glycine-NaCl-NaOH
Figure 6. Thermal stability; 15-min. treatment with 50 mM K-phosphate buffer, pH 7.5
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: C5896-100UN
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: C5896-1KU
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: C5896-50UN
| form | lyophilized powder |
| mol wt | ~95 kDa |
| Quality Level | 200  |
| specific activity | ≥10 units/mg solid |
| storage temp. | −20°C |
| suitability | suitable for assay of choline |
| suitable for molecular biology | |
| technique(s) | cell based assay: suitable |
| Application: | Choline oxidase from Alcaligenes sp. has been used:• in a study to investigate a choline biosensor constructed with a chitinous membrane and its application in measuring cholinesterase inhibitory activities • has also been used in spectroscopic studies on the photoreaction of choline oxidase with covalently bound flavin. • as a component of phosphate buffer saline (PBS) buffer for measurement of choline acetyltransferase (ChAT) activity in brain tissue homogenates • to measure the choline generated by autotaxin (ATX) by ATX activity assay and phospholipase-D (PLD) by PLD activity assay |
| Biochem/physiol Actions: | Choline oxidase catalyzes the four-electron oxidation of choline to glycine-betaine, with betaine-aldehyde as the intermediate and molecular oxygen as th eprimary electron acceptor. The enzyme can also accept betaine-aldehyde as a substrate. This allows the study of the reaction mechanism for the conversion of choline to the aldehyde intermediate, & of betaine-aldehyde to glycine-betaine. The enzyme is a flavoprotein with a molecular weight of approx. 72,000 Da (gel filtration) or 66,000 Da (SDS gel electrophoresis). |
| General description: | Isoelectric point: 4.1 ± 0.1 Michaelis constants: 2.84 x 10¯3M (Choline), 5.33 x 10¯3M (Betaine aldehyde) Structure: One mol of FAD is covalently bound to mol of the enzyme Inhibitors: p-Chloromercuribenzoate, Cu++, Co++, Hg++, Ag+ Optimum pH: 8.0 – 8.5 Optimum temp: 40 – 45°C pH Stability: pH 7.0 – 9.0 (30°C, 2hr) Thermal stability: Below 37°C (pH 7.5, 10min) |
| Other Notes: | One unit will form 1 μmole of H2O2 with oxidation of 1 μmole of choline to betaine aldehyde per min at pH 8.0 at 37 °C. Note: During the conversion of choline to betaine by choline oxidase, 2 μmoles of H2O2 are produced for every μmole of choline. |
| Packaging: | 100, 500, 1000 units in poly bottle |
| Packaging: | 50 units in glass bottle |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H334 |
| Precautionary statements | P261 - P284 - P501 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥10 units/mg solid |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 1.1.3.17 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |