Anti-Cytokeratin, pan antibody, Mouse monoclonal
SIGMA/C5992 - clone PCK-26, purified from hybridoma cell culture
Synonym: Monoclonal Anti-pan-Cytokeratin; Anti-Cytokeratin; Anti-Cytokeratin - Monoclonal Anti-Cytokeratin, pan antibody produced in mouse
Product Type: Chemical
Immunohistochemistry Formalin-fixed, paraffin-embedded tissue section of human placenta stained with 20 μg/mL Monoclonal Anti-Cytokeratin, pan antibody produced in Mouse clone PCK-26 (Cat. No. C5992) followed by Anti-Mouse IgG (Fab specific-Biotin antibody produced in goat (Cat. No. B0529) and by ExtrAvidin®-Peroxidase (Cat. No. E2886).
Immunohistochemistry Formalin-fixed, paraffin-embedded tissue section of human skin stained with 20 μg/mL Monoclonal Anti-CytokeRatin, pan antibody produced in Mouse clone PCK-26 (Cat. No. C5992) followed by Anti-Mouse IgG (Fab specific)-Biotin antibody produced in Goat (Cat. No. B0529) and by ExtrAvidin®-Alkaline Phosphatase (Cat. No. E2886).
Immunohistochemistry Formalin-fixed, paraffin-embedded tissue section of human placenta stained with 20 μg/mL Monoclonal Anti-CytokeRatin, pan antibody produced in Mouse clone PCK-26 (Cat. No. C5992) followed by Anti-Mouse IgG (Fab specific)-FITC antibody produced in Goat (Cat. No. F5262).
Immunoblotting Whole extract of HeLa cells was separated on SDS-PAGE and probed with Monoclonal Anti-Cytokeratin, pan Clone: PCK-26 (Cat. No. C5992). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. Antibody dilution 2.5 μg/mL 2. Antibody dilution 5 μg/mL 3. Antibody dilution 10 μg/mL
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | PCK-26, monoclonal |
| concentration | ~1.5 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| packaging | antibody small pack of 25 μL |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human, chicken, snake, hamster, pig, goat, feline, bovine, carp, rat, rabbit, canine, lizard, sheep, mouse, guinea pig |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | dot blot: suitable |
| immunocytochemistry: suitable | |
| immunohistochemistry: 10-20 μg/mL using human placenta or skin | |
| western blot: suitable |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Immunofluorescence (1 paper) |
| Application: | Monoclonal Anti-Cytokeratin, pan antibody produced in mouse has been used in: • immunoblotting • immunohistochemistry • Immunocytochemistry • dot blotting |
| Biochem/physiol Actions: | Cytokeratin facilitates the typing of normal, metaplastic, and neoplastic cells and may aid in the discrimination of carcinomas and non-epithelial tumors such as sarcomas, lymphomas, and neural tumors. It is also useful in detecting micrometastases in lymph nodes and other tissues, and for determining the origin of poorly differentiated tumors. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-Pan Cytokeratin (mouse IgG1 isotype) is derived from the PCK-26 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a cytokeratin preparation from human epidermis. Cytokeratins are a group of at least 29 different proteins. They are characteristic of epithelial and trichocytic cells. Cytokeratin peptide 1 (68 kDa) is expressed together with cytokeratin 10 in the suprabasal cell layers or the differentiation compartment of the epidermis. Its expression increases with epidermal maturation and is modified post translationally in terminally differentiated keratinocytes of the stratum corneum. Cytokeratin peptide 5 (58 kDa) is the primary type II keratin in stratified epithelia, while cytokeratin type 8 (52 kDa) is a major type II keratin in simple epithelia. Cytokeratin 6 (56 kDa) is a "hyperproliferation" cytokeratin expressed in tissues with natural or pathological high turnover. Monoclonal antibodies to cytokeratins are specific markers of epithelial cell differentiation and have been widely used as tools in tumor identification and classification. |
| Immunogen: | cytokeratin from human epidermis. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Specificity: | The antibody recognizes an epitope located on the Type II cytokeratins 1, 5, 6, and 8. PCK-26 is a broad spectrum antibody which reacts specifically with a variety of normal, reactive, and neoplastic epithelial tissues. The antibody reacts with simple, cornifying, and non-cornifying squamous epithelia and pseudostratified epithelia. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |