Carbonic Anhydrase Isozyme II human
SIGMA/C6165 - ≥80%, powder, ≥3,000 W-A units/mg protein
Synonym: Carbonate Dehydratase; Carbonate Hydrolyase
CAS Number: 9001-03-0
EC Number: 232-576-6
MDL Number: MFCD00081467
Product Type: Chemical
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| assay | ≥80% |
| color | white |
| form | powder |
| mol wt | 30 kDa |
| pI | ~7.4 |
| Quality Level | 200  |
| specific activity | ≥3,000 W-A units/mg protein |
| storage temp. | −20°C |
| UniProt accession no. | P00918  |
| Application: | Human carbonic anhydrase isozyme II has been used to assess its gene fusion abilities for efficient expression and recovery of recombinant proteins. Human carbonic anhydrase isozyme II has also been used to investigate a new process for the synthesis of difluoromethanesulfonamid |
| Biochem/physiol Actions: | Carbonic anhydrase is a zinc metalloenzyme that catalyzes the hydration of carbon dioxide to carbonic acid. It is involved in pathological and physiological processes such as pH and CO2 homeostasis, transport of bicarbonate and CO2, biosynthetic reactions, bone resorption, calcification, and tumorigenicity. Therefore, this enzyme is an important target for inhibitors that can be used for treating various pathological conditions such as glaucoma, epilepsy and Parkinson’s disease. CAII has a molecular mass of approximately 30 kDa and is primarily present in type II pneumocytes. Due to this unique location it has been speculated that CAII is involved in pulmonary functions such as regulation of fluid secretion and CO2 elimation. Sulfonamides, sulfamates and sulfamides are potent inhibitors of CA. |
| Other Notes: | One Wilbur-Anderson (W-A) unit will cause the pH of a 0.02 M Trizma buffer to drop from 8.3 to 6.3 per min at 0 °C. (One W-A unit is essentially equivalent to one Roughton-Booth unit.) |
| Packaging: | 1, 5, 25 mg in glass bottle |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥80% |
| activity | specific activity: ≥3,000 W-A units/mg protein |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 4.2.1.1 ( BRENDA | IUBMB ) |
| UNSPSC | 12352204 |