Cathepsin B from bovine spleen
SIGMA/C6286 - lyophilized powder, ≥10 units/mg protein
Synonym: Cathepsin B1
CAS Number: 9047-22-7
MDL Number: MFCD02662345
Product Type: Chemical
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| biological source | bovine spleen |
| composition | Protein, ≥30% biuret |
| form | lyophilized powder |
| Quality Level | 200  |
| specific activity | ≥10 units/mg protein |
| storage temp. | −20°C |
| UniProt accession no. | P07688  |
| Application: | Cathepsin B from bovine spleen has been used in cleavage assay before high-performance liquid chromatography-mass spectrometry (HPLC-MS) analysis. It has also been used in the enzyme inhibition assays with protease inhibitors RfIP1 and ruthenium metalloarenes. |
| Biochem/physiol Actions: | Cathepsin B displays an endopeptidase and peptidyl dipeptidase activities. It may be associated with the pathophysiology of tumors. Cathepsin B is also regarded as a prominent protease in Leishmaniasis. It is overactivated in muscular dystrophy, pulmonary emphysema, and bone resorption. |
| Biochem/physiol Actions: | Cathepsin B has been found to cleave procaspase 1 and procaspase 11 and to induce apoptosis in digitonin-permeabilized cells. Translocation of cathepsin B from the cytoplasm to the nucleus contributes to bile salt induced apoptosis of rat hepatocytes. Levels of cathepsin B in PC12 cells significantly decrease 12 to 24 hours after apoptosis is induced. |
| Biochem/physiol Actions: | Found to cleave procaspase 1 and procaspase 11 and to induce apoptosis in digitonin-permeabilized cells. |
| General description: | Cathepsin B is a lysosomal enzyme that comprises a light chain (Lys1–Arg49) and a heavy chain (Val50–Thr253). It belongs to cysteine family C1. |
| Other Notes: | One unit will hydrolyze 1 μmole of Z-lysine p-nitrophenyl ester per min at pH 5.0 at 25 °C. |
| Packaging: | 10, 25, 100 units in serum bottle |
| Physical form: | Lyophilized powder containing sodium phosphate, sodium chloride and ~6% EDTA as stabilizer. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥10 units/mg protein |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 3.4.22.1 ( BRENDA | IUBMB ) |
| UNSPSC | 12352204 |