Monoclonal Anti-Connexin-32 antibody produced in mouse
SIGMA/C6344 - clone CXN-32, ascites fluid, buffered aqueous solution
MDL Number: MFCD00803531
Product Type: Chemical
Immunoblotting Mouse brain extract was separated on SDS-PAGE and probed with Monoclonal AB Connexin 32 (MO) Clone: CXN-32 (Cat. No. C6344). The antibody was developed with Al-Hh APA to Mouse IgG (Fab)(Gt) (Cat. No. A1293) and a BCIP/NBT substrate. Antibody dilution 1/1,000.
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | CXN-32, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen 27 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human, rat, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (frozen sections): suitable |
| indirect ELISA: suitable | |
| microarray: suitable | |
| western blot: 1:1,000 using a mouse whole brain extract | |
| UniProt accession no. | P08034  |
| Application: | Monoclonal Anti-Connexin-32 antibody produced in mouse has been used in: • enzyme linked immunosorbent assay (ELISA) • immunoblotting • epifuorescent microscopy |
| Biochem/physiol Actions: | Connexin-32 low expression levels is observed in common bile duct ligation (CBDL). In addition, a combination of myelin disruption and axonal degeneration has been shown to occur with Cx32 mutations in Charcot-Marie-Tooth disease (CMTX). Monoclonal antibodies reacting specifically with Cx32, may be used in diverse cellular and molecular approaches to the study of gap junctions and their properties, and to correlate their expression pattern with physiological functions or pathological conditions. |
| Biochem/physiol Actions: | Connexins belong to the gap junction protein family. In humans, connexin is encoded by GJB1 (gap junction protein, β 1) gene. The gap junction proteins connexin32 (Cx32), Cx37, Cx40 and Cx43 are expressed in endothelial cells and regulate vascular functions involving inflammation. The endothelial Cx32 positively regulates angiogenesis by enhancing endothelial cell tube formation and cell migration. CX32 mutation is associated with cognitive impairment and a differential degree of peripheral nerve involvement. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-Connexin-32 (mouse IgG1 isotype) is derived from the CXN-32 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. The 27 kDa connexin protein (Connexin-32, Cx32), belongs to the connexion family of proteins. It is expressed in most tissues, even though the pattern of expression may differ in various cell types including peripheral and central nervous system. |
| Immunogen: | synthetic connexin-32 peptide (amino acids 105-123). |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |