α-Chymotrypsin from bovine pancreas
SIGMA/C6423 - suitable for protein sequencing, salt-free, lyophilized powder
CAS Number: 9004-07-3
EC Number: 232-671-2
MDL Number: MFCD00130481
Product Type: Chemical
α-Chymotrypsin is a serine protease of the peptidase S1 family consisting of 241 amino acid residues. The molecule has three peptide chains: an A chain of 13 residues, a B chain of 131 residues, and a C chain of 97 residues. α-Chymotrypsin is the predominant form of active enzyme produced from its zymogen, Chymotrypsinogen A.
| form | salt-free, lyophilized powder |
| grade | Proteomics Grade |
| mol wt | 25 kDa |
| Quality Level | 200  |
| storage temp. | 2-8°C |
| suitability | suitable for protein sequencing |
| UniProt accession no. | P00767  |
| Application: | α-Chymotrypsin from bovine pancreas is used for the following applications: • Protein Identification by mass spectrometry (MS) • The isolation and characterization of myosin heavy chains • Toxin preparation • The incubation of infected and uninfected cells for analysis of cellular proteins by SDS-PAGE α-Chymotrypsin from bovine pancreas (BPC) may be used as a catalyst in the preparation of tetrahydroquinoline derivatives by Povarov reaction. |
| Biochem/physiol Actions: | A serine protease that hydrolyzes peptide bonds with aromatic or large hydrophobic side chains (Tyr, Trp, Phe, Met, Leu) on the carboxyl end of the bond. |
| Biochem/physiol Actions: | α-Chymotrypsin from bovine pancreas is stabilized by Ca2+ and catalyzes the hydrolysis of peptide bonds in particular the amino acids tyrosine, phenylalanine, tryptophan, and leucine at their C-terminal side. α-Chymotrypsin is inhibited by Cu2+ and Hg. |
| General description: | Chymotrypsin (Chy) is a serine protease. It corresponds to a molecular weight of 25.7 kDa and is widely used in pharmaceutical industry. It is synthesized in pancreas from chymotrypsinogen and require calcium for this conversion. |
| General description: | α-Chymotrypsin from bovine pancreas (bovine pancreatic α-chymotrypsin, CHT) is an enzyme protein. The influence of varying concentrations of organic solvents like ethanol, 1,4-dioxane and acetonitrile on CHT has been reported. |
| Other Notes: | One unit will hydrolyze 1.0 μmole of BTEE per min at pH 7.8 at 25 °C. |
| Other Notes: | View more information on chymotrypsin at www.sigma-aldrich.com/enz |
| Symbol |    GHS07,GHS08,GHS09 |
| Signal word | Danger |
| Hazard statements | H302 - H315 - H319 - H334 - H335 - H400 |
| Precautionary statements | P261 - P273 - P301 + P312 - P302 + P352 - P304 + P340 + P312 - P305 + P351 + P338 |
| Hazard Codes | Xn |
| Risk Statements | 36/37/38-42 |
| Safety Statements | 22-24-26-36/37 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 3.4.21.1 ( BRENDA | IUBMB ) |
| UNSPSC | 12352204 |