Anti-Cdc7 Kinase antibody, Mouse monoclonal
SIGMA/C6613 - clone DCS-341, purified from hybridoma cell culture
MDL Number: MFCD04975117
Product Type: Chemical
Immunoblotting Whole extract of A431 cells was separated on SDS-PAGE, blotted with 0.4 μg/mL Monoclonal Anti-Cdc7 Kinase Clone: DCS-341 (Cat. No. C6613) and developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | DCS-341, monoclonal |
| concentration | ~2 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: suitable |
| immunoprecipitation (IP): suitable | |
| indirect ELISA: suitable | |
| microarray: suitable | |
| western blot: 0.4 μg/mL using total cell extract of A431 cells | |
| UniProt accession no. | O00311  |
| Application: | Anti-Cdc7 Kinase antibody, Mouse monoclonal has been used in: • enzyme-linked immunosorbent assay (ELISA) • immunoblotting • immunoprecipitation • immunocytochemistry. |
| Biochem/physiol Actions: | Cell division cycle 7-related protein kinase (CDC7) in addition to its role in chromosome structure maintenance and repair, is also involved in meiosis and checkpoint responses. |
| Biochem/physiol Actions: | Cell division cycle 7-related protein kinase(CDC7) is a serine-threonine essential kinase required for the initiation of DNA replication and is involved in regulation of the cell cycle. It is high expressed in diffuse large B cell lymphoma (DLBCL). Loss of Cdc7 kinase expression results in retarded growth at both cellular and whole body levels, with especially profound impairment of germ cell development. It inhibits genotoxin-induced apoptosis and increases survival in cancer cells. It may play an important role in the development and progression of colorectal cancer. Depletion of Cdc7 induces cancer cell death regardless of its p53 status. It can also interact with and phosphorylate Claspin. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Anti-Cdc7 Kinase antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the DCS-341 hybridoma produced by the fusion of mouse myeloma cells (NS2) and splenocytes from BALB/c mice immunized with full length human Cdc7 kinase. Cell division cycle 7-related protein kinase(CDC7) is an enzyme encoded by the CDC7 gene in humans. It is highly expressed in diffuse large B cell lymphoma (DLBCL). Human Cdc7 kinase consists of 574 amino acids with a molecular weight of 55 kDa. CDC7 shares nearly 45% identity with yeast Cdc7 kinase in the kinase domain. |
| Immunogen: | full-length human Cdc7 kinase. |
| Packaging: | 200 μL in glass insert |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |