4-Chloro-1-naphthol
SIGMA/C6788 - tablet
Synonym: 4-CN; 4-
CAS Number: 604-44-4
Empirical Formula (Hill Notation): C10H7ClO
Molecular Weight: 178.61
EC Number: 210-068-5
MDL Number: MFCD00003974
Linear Formula: ClC10H6OH
Product Type: Chemical
| form | tablet |
| InChI | 1S/C10H7ClO/c11-9-5-6-10( |
| InChI key | LVSPDZAGCBEQAV-UHFFFAOYSA |
| mp | 118-121 °C (lit.) |
| Quality Level | 200  |
| SMILES string | Oc1ccc(Cl)c2ccccc12 |
| solubility | methanol: 10 mL, clear, colorless |
| storage temp. | −20°C |
| Application: | 4-Chloro-1-naphthol has been used as a substrate in enzyme-linked immunospot (ELISpot) assay for detection of the spots. It has also been used as a precipitating chromogenic substrate for visualizing the antigen−antibody-reactive bands following western blotting. |
| Biochem/physiol Actions: | 4-Chloro-1-Naphthol is a peroxidase substrate suitable for use in immunoblotting procedures. This substrate produces an insoluble end product that is blue in color and can be observed visually. |
| Preparation Note: | Contains 30 mg substrate per tablet. |
| Preparation Note: | Dissolve 1 tablet in 10 ml of methanol. Add 2 ml of methanol stock solution to 10 ml of triethanolamine buffered saline, pH 7.5. Add 5 μl of fresh 30% hydrogen peroxide (Sigma Product No. H-1009) immediately prior to use. |
| Hazard Codes | Xi |
| Risk Statements | 36/37/38 |
| Safety Statements | 26-36 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| mp | 118-121 °C (lit.) |
| Storage Temp. | −20°C |
| UNSPSC | 12352204 |