Monoclonal Anti-Collagen, Type VII antibody produced in mouse
SIGMA/C6805 - clone LH7.2, ascites fluid
Synonym: Anti-EBD1; Anti-EBDCT; Anti-EBR1; Anti-NDNC8
MDL Number: MFCD00164570
Product Type: Chemical
Immunohistochemistry Formalin-fixed, paraffin-embedded heat retrieval tissue section of human tonsil stained with 1:1,000 Monoclonal Anti-Collagen, Type VII antibody produced in Mouse clone LH7.2 (Cat. No. C6805) followed by Anti-Mouse IgG (Fab specific)-FITC antibody produced in Goat (Cat. No. F5262).
Immunohistochemistry Frozen acetone fixed human tonsil sections stained with 1:1,000 Monoclonal Anti-Collagen, Type VII antibody produced in Mouse clone LH7.2 (Cat. No. C6805) followed by Anti-Mouse IgG (Fab specific)-FITC antibody produced in Goat (Cat. No. F5262).
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | LH7.2, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| isotype | IgG1 |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human, goat, marmoset, monkey, guinea pig, sheep, pig, bovine |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | dot blot: suitable |
| immunocytochemistry: suitable | |
| indirect ELISA: suitable | |
| indirect immunofluorescence: 1:1,000 using human or other mammalian frozen sections | |
| western blot: suitable | |
| UniProt accession no. | Q02388  |
| Application: | Monoclonal anti-collagen type VII antibody can be used as a primary antibody in virus titration. It can also be used for the localization of type VII collagen in different immunochemical assays like dot blot, immunocytochemistry and ELISA. |
| Application: | Monoclonal Anti-Collagen, Type VII antibody produced in mouse has been used in immuno-gold labelling. |
| Biochem/physiol Actions: | Collagen type VII alpha 1 chain (COL7A1) is required for epithelium-to-stroma anchorage in skin, mucosa and cornea. It is considered as an immediate-early response gene for transforming growth factor β (TGF-β)/SMAD (mothers against decapentaplegic) signaling pathway. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Collagen is a fibrous protein present in the extracellular framework of all vertebrates. Collagen type VII is the crucial component of anchoring fibrils and mutation in type VII collagen gene (COL7A1) leads to epidermolysis bullosa dystrophica. Monoclonal anti-collagen, type VII antibody can be used to distinguish invasive melanoma from non-invasive melanoma by visualizing the appearance and integrity of epidermal basement membrane. It can also be used in immunoblotting. Mouse anti-collagen type VII antibody reacts specifically with an epitope located on collagenase digested type VII collagen (150 kDa). The product also reacts with the basement membrane zone of stratified squamous epithelia of sheep, pig, bovine, guinea pig, goat and human. |
| General description: | Collagen type VII alpha 1 chain (COL7A1) is majorly expressed in skin, secreted by keratinocytes and fibroblasts. The gene is located on human chromosome 3p21.31. |
| Immunogen: | insoluble fractions of neonatal human foreskin epidermal cells |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |