Anti-CCRK (381-395) antibody produced in rabbit
SIGMA/C6869 - IgG fraction of antiserum, buffered aqueous solution
Synonym: Anti-CDCH; Anti-Cell cycle related kinase isoform 2 (Homo sapiens); Anti-p42
Product Type: Chemical
Immunofluorescence Anti-CCRK (381-395) (RB): Cat. No. C6869: Immunofluorescence of HUVEC cells using CCRK (381-395) (RB), Cat. No. C6869 (red) at a 1:100 dilution, taken at 40× magnification and nuclear staining with Hoescht 33342 (blue). Yale HTCB IF procedure used. Images have been adjusted to improve viewing quality. If you would like the original image, please send a request to protocols@sial.com
Immunoblotting Lysate of HEK-293T cells overexpressing human CCRK was separated on SDS-PAGE, blotted with Anti-CCRK (381-395) (Cat. No. C6869) and developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. Antibody dilution 1:500 2. Antibody dilution 1:500 + CCRK immunizing peptide (human, 381-395)
Chart Anti-CCRK (381-395) (RB): Cat. No. C6869: Intensity analysis of Anti-CCRK (381-395) (RB) staining. Cytoplasmic and nuclear intensity values were obtained at 8 different antibody dilutions (1:50 to 1:6,400) as compared with the negative control. Yale HTCB IF procedure used.
| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~36 kDa |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | western blot: 1:500-1:2,000 |
| Application: | Yale Center for High Throughput Cell Biology IF-tested antibodies. Each antibody is tested by immunofluorescence against HUVEC cells using the Yale HTCB IF protocol. To learn more about us and Yale Center for High Throughput Cell Biology partnership, visit sigma.com/htcb-if  . Anti-CCRK (381-395) antibody produced in rabbit is suitable for western blotting at a dilution of 1:500-1:2,000. |
| Biochem/physiol Actions: | Cell cycle-related kinase (CCRK) is a protein kinase that is homologous to Cdk7. It is a candidate for oncogene in human glioblastoma tumorigenesis. CCRK is also involved in colorectal cancer carcinogenesis and G1/S cell cycle transition by regulating Cdk2, cyclin E and Rb. CCRK plays an important role in the control of cell proliferation via the regulation of cyclin D1 expression, and the overexpression of CCRK. It also acts as an independent molecular marker for shortened survival time of patients with ovarian carcinoma. CCRK along with its substrate ICK (intestinal cell kinase) inhibits ciliogenesis and glioblastoma cell proliferation. Depletion of CCRK leads to accumulation of ICK at ciliary tips, altered ciliary transport and leads to inhibition of cell cycle re-entry in NIH3T3 fibroblasts. |
| Immunogen: | synthetic peptide corresponding to amino acids 381-395 of human CCRK. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |