Anti-Cortactin (GK-18) antibody produced in rabbit
SIGMA/C6987 - IgG fraction of antiserum, buffered aqueous solution
Synonym: Anti-EMS1; Anti-p80/85
MDL Number: MFCD03791486
Product Type: Chemical
Immunofluorescence NIH-3T3 Mouse fibroblasts cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton X-100. Fixed cells were stained with 1:500 Anti-Cortactin (GK-18) antibody produced in Rabbit (Cat. No. C6987) followed by 1:500 Anti-Rabbit IgG-FITC.
Western Blotting Whole extract of A431 cells were separated on SDS-PAGE and probed with Anti-Cortactin (GK-18) antibody produced in Rabbit (Cat. No. C6987). The antibody was developed using 1:10,000 Anti-Rabbit IgG (whole molecule)-Peroxidase antibody produced in Goat (Cat. No. A0545). Lanes 1. 1:1,000 antibody 2. 1:2,000 antibody 3. Negative Control
| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human, mouse, rat, chicken |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect immunofluorescence: 1:500 using mouse fibroblasts NIH3T3 cell line |
| microarray: suitable | |
| western blot: 1:1,000 using cytosol extract of rat brain, whole extract of chicken embryo fibroblasts, or whole cell extract of human epidermoid carcinoma A431 cell line | |
| UniProt accession no. | Q14247  |
| Application: | Anti-Cortactin (GK-18) antibody produced in rabbit is suitable for the following applications: • Indirect immunofluorescence at a dilution of 1:500 using mouse fibroblasts NIH3T3 cell line • Microarray • Western blotting at a dilution of 1:1,000 using cytosol extract of rat brain, whole extract of chicken embryo fibroblasts or whole cell extract of human epidermoid carcinoma A431 cell line |
| Biochem/physiol Actions: | Cortactin (EMS1) is an important branched actin regulator involved in invasion and migration of tumor cells and hepatocellular carcinoma (HCC). Overexpression of this protein is closely associated with poor human HCCs prognosis caused by cancer embolus and metastasis. The overexpressed protein promotes vesicular trafficking at a variety of cellular sites including endosomes and the trans-Golgi network. Trafficking is highly sensitive to cortactin-regulated branched actin assembly. |
| Biochem/physiol Actions: | Cortactin is overexpressed in human breast cancer and in head and neck tumors. Overexpression and redistribution of cortactin into podosome-like structures has been observed in human carcinoma cell lines suggesting that cortactin is involved in tumor cell invasion and metastasis. The C-terminal region contains a Src-homology 3 (SH3) domain that interacts with several postsynaptic density (PSD), PDZ-family (post-synaptic density protein 95 (PSD-95), Drosophila disc large tumor suppressor (Dlg1) and zona occludens 1 (ZO-1)) proteins) including cortactin-binding protein 1 (CortBP1), SH3 and multiple ankyrin repeat domains 3 (SHANK3), Zonula occludens-1 (ZO-1) and Cortactin-binding protein 90 (CBP90) (CBP-90). Cortactin is enriched within lamellipodia of motile cells and in neuronal growth cones. Cortactin is normally phosphorylated on serine and threonine residues. Cortactin becomes heavily phosphorylated on tyrosine in pp60src transfected oncogenic fibroblasts and accumulates with F-actin in podosomes. Cortactin co-localizes with the actin-related protein Arp2/3 complex at sites of actin polymerization within the lamellipodia It binds directly to the Arp2/3 complex and activates it to promote assembly of branched actin filaments networks. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Cortactin gene is located on human chromosome 11q13. Cortactin exists as multiple isoforms (75-85 kDa), formed by alternative splicing. It contains an unique multidomain structure consisting of 6.5 tandem repeats of a 37-amino acid sequence required for direct binding to F-actin, followed by an α-helix region, a proline-rich region, and three tyrosine residues targeted by sarcoma (Src) related kinases. It represents a highly conserved family of perimembrane signaling proteins, including the hematopoietic lineage cell specific proteins hematopoietic cell-specific Lyn substrate 1 (HS1/LCKBP1) and hematopoietic progenitor kinase 1 [HPK1]-interacting protein of 55 kDa (HIP-55). |
| Immunogen: | synthetic peptide corresponding to the N-terminal region of human cortactin (amino acids 18-35) conjugated to KLH. |
| Physical form: | Supplied as a solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |