Monoclonal Anti-CD3 antibody produced in mouse
SIGMA/C7048 - clone UCHT-1, purified immunoglobulin, buffered aqueous solution
Synonym: Monoclonal Anti-CD3
Product Type: Chemical
Immunohistochemistry
Frozen section of human tonsil stained with Monoclonal AntiCD3, clone no. UCHT-1 (Cat. No. C7048) using the APAAP method.
From K. Miller, University College and Middlesex School of Medicine, London.
Frozen section of human tonsil stained with Monoclonal AntiCD3, clone no. UCHT-1 (Cat. No. C7048) using the APAAP method.
From K. Miller, University College and Middlesex School of Medicine, London.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | UCHT-1, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| Quality Level | 200  |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | flow cytometry: 5 μL using 1 × 106 cells |
| UniProt accession no. | P07766  |
| Application: | Monoclonal Anti-CD3 antibody produced in mouse is suitable for flow cytometry at a dilution of 5μL using 1 × 106 cells. |
| Biochem/physiol Actions: | Recognizes the CD3 complex. The epitope recognized by clone no. UCHT-1 is expressed on the ε-chain of the CD3 antigen/T cell receptor complex. 3rd Workshop: code no. 126, 208 and 471 4th Workshop: paper no. T3.2 |
| Biochem/physiol Actions: | The CD3e cytoplasmic domain of the T cell receptor binds to the inner leaflet of the plasma membrane (PM) and is accessible during early stages of T cell activation. It is specific for T lymphocytes, found in Purkinje cells of the cerebellum and gastric parietal cells of humans and other species. The antibodies commonly used to recognize CD3 are directed against the ε-subunit of the T cell receptor. Cytoplasmic cyCD3 expression can only be achieved during the early phase of NK-cell differentiation. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | The CD3 complex (16/20/29/22/25-28 kDa) consists of 5 polypeptide chains associated with each other and with the T cell receptor. |
| Immunogen: | human thymocytes followed by Sezary T cells. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |