Monoclonal Anti-β-Catenin antibody produced in mouse
SIGMA/C7082 - clone 6F9, ascites fluid
Synonym: Anti-CTNNB; Anti-EVR7; Anti-MRD19; Anti-NEDSDV; Anti-armadillo
MDL Number: MFCD00282178
Product Type: Chemical
Immunofluorescence MDCK cells were fixed and permeabilized with cold methanol followed by cold acetone. Fixed cells were stained with 1:500 Monoclonal Anti-β-Catenin Clone: 6F9 (Cat. No. C7082). The antibody was developed using Goat Anti-Mouse IgG, Atto-488 conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.
Immunoblotting Cell line lysates were separated on SDS-PAGE and probed with 1:500 Monoclonal Anti-β-Catenin Clone: 6F9 (Cat. No. C7082). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. HeLa 2. MBDK 3. MDCK 4. NRK 5. 3T3 6. Chicken fibroblasts
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 6F9, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| isotype | IgG1 |
| mol wt | antigen 94 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human, chicken, canine, bovine |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect immunofluorescence: 1:1,000 using cultured MDBK cells |
| microarray: suitable | |
| western blot: 1:1,000 using cultured MDBK cells extract | |
| UniProt accession no. | P35222  |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Immunofluorescence (1 paper) |
| Application: | Monoclonal Anti-β-Catenin antibody produced in mouse has been used in: • immunohistochemistry • immunofluorescence • immunoblotting |
| Biochem/physiol Actions: | In Wnt/β-catenin signal pathway, β-Catenin participates as a signal transduction molecule. The pathway is associated with development, tissue homeostasis and oncogenic transcription. Upregulation of the gene is associated with glioma cell proliferation. The encoded protein has dual function that regulates the coordination of cell-cell adhesion by linking cytoskeleton and cellular junctions and gene transcription. Wnt/β-catenin signal pathway is known to induce progression of colon cancer progression. It is useful for differential diagnosis of Osteoblastoma (OB) and osteosarcoma (OS). β-catenin signaling pathway may contribute to cholangiocarcinoma (CCA) cell proliferation. Monoclonal antibody reacting specifically with β -catenin is an essential tool in defining the interactions and distributions of β -catenin and its relationships with other catenins and cadherins in various cells and tissues. |
| Biochem/physiol Actions: | The antibody does not cross-react with plakoglobin (γ-catenin). |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti- β -Catenin (mouse IgG1 isotype) is derived from the 6F9 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. CTNNB1 (catenin β 1) gene is mapped to human chromosome 3p21. The protein encoded is also called as cadherin-associated protein.The encoded protein β-catenin is localized to the nucleus. Monoclonal antibody reacting specifically with β -catenin is an essential tool in defining the interactions and distributions of β -catenin and its relationships with other catenins and cadherins in various cells and tissues. |
| Immunogen: | recombinant chicken β-catenin. |
| Physical form: | Supplied as ascites fluid with 15 mM sodium azide as a preservative. |
| Preparation Note: | For extended storage freeze in working aliquots. For continuous use, store at 2-8 °C. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |