Monoclonal Anti-Cdk7/CAK antibody produced in mouse
SIGMA/C7089 - clone MO-1.1, ascites fluid
MDL Number: MFCD00282167
Product Type: Chemical
Immunoblotting A431 cell extract was separated on SDS-PAGE and probed with Monoclonal AB TO CAK (CDk7) (MO) CL:MO-1.1 (Cat. No. C7089). The antibody was developed with Alk Phos APA to Mouse IgG (Fab) (Gt) (Cat. No. A1293) and a NBT/BCIP substrate. Antibody dilution 1/50,000.
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | MO-1.1, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| isotype | IgG2b |
| mol wt | antigen 40 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: suitable |
| immunohistochemistry (frozen sections): suitable | |
| immunoprecipitation (IP): suitable | |
| microarray: suitable | |
| western blot: 1:50,000 using a cultured human cell line extract | |
| UniProt accession no. | P50613  |
| Application: | Monoclonal Anti-Cdk7/CAK antibody produced in mouse is suitable for the following applications: • Immunocytochemistry • Immunohistochemistry (frozen sections) • Immunoprecipitation • Microarray • Western blotting at a dilution of 1:50,000 using a cultured human cell line extract |
| Biochem/physiol Actions: | Cyclin-dependent kinase (CDK) Activating Kinase (CAK) is involved in activation and phosphorylation of CDK1, CDK2, CDK4 and CDK6 and regulation of the cell cycle. It is required for cell cycle progression and it could be a target for cancer therapy. CAK plays a central role in regulation of messenger RNA synthesis by either inhibition of RNA polymerase II-catalyzed transcription or stimulation of transcription through association with basal transcription repair factor IIH. Phosphorylation by the CDK-activating kinase (CAK) is required for the activation of cyclin-dependent kinases. |
| Biochem/physiol Actions: | The antibody reacts specifically with Cdk7 (40 kDa catalytic subunit of Cdk activating kinase). |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Cell division protein kinase 7 is an enzymes encoded by the CDK7 gene in humans. The protein belongs to cyclin-dependent protein kinase (CDK) family. CAK is a CDK-cyclin complex implicated in the control of multiple cell cycle transitions. The structure of CDK7 facilitates the inhibitors with differential specificity for the different CDKs and can be used in different biological processes. |
| Immunogen: | recombinant human Cdk7 protein. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |