Anti-Calcitonin Gene-Related Peptide antibody, Mouse monoclonal
SIGMA/C7113 - clone 4901, purified from hybridoma cell culture
Synonym: Anti-CALC; Anti-CCALCI; Anti-CGRP-I
MDL Number: MFCD00162203
Product Type: Chemical
ELISA Monoclonal Anti-Calcitonin Gene-Related Peptide antibody produced in Mouse (Cat. No. C7113), followed by 1:1,000 Anti-Mouse IgG (Fab specific)-Peroxidase antibody produced in Goat (Cat. No. A9917), was serially diluted to detect CGRP (Calcitonin gene-related peptide) by indirect ELISA. Reactivity is observed with Rat α-CGRP, Human α-CGRP and Human β-CGRP.
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 4901, monoclonal |
| concentration | ~2 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| packaging | antibody small pack of 25 μL |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | rat, human, canine |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: suitable |
| indirect ELISA: 0.5 μg/mL using rat α-CGRP | |
| neutralization: suitable | |
| radioimmunoassay: suitable | |
| UniProt accession no. | P01258  |
| Application: | Monoclonal anti-calcitonin gene-related peptide antibody can be used in immunocytochemistry and immunohistochemistry. It can also be used in ELISA. |
| Application: | Anti-Calcitonin Gene-Related Peptide (CGRP) antibody is suitable for ELISA at a working concentration of 0.25- 0.5 μg/ml. The antibody may be used for immunofluorescence at a working dilution of 1:100 in rat nerve fibres and human dorsal root ganglia. |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Immunofluorescence (1 paper) Immunohistochemistry (1 paper) |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Calcitonin gene-related peptide (CGRP) is a vasodilator that belongs to the calcitonin family of peptides. It is produced by motor neurons of peripheral and central nervous system. There are two forms of CGRP, α and β, which exhibit similar functions. The effects of CGRP are mediated by G-protein coupled receptor heterodimer formed by calcitonin-receptor-like receptor (CRLR) and receptor-activity-modifyi Monoclonal Anti-Calcitonin Gene-Related Peptide (CGRP) recognizes rat α-CGRP. The antibody cross-reacts with human and dog α- and β-CGRP. |
| General description: | CGRP (Calcitonin gene-related peptide) belongs to calcitonin family and is expressed mainly in the central and peripheral nervous systems. It is a strong endogenous vasodilator. CGRP concentration increases in migraine attacks and hence, it is involved in the pathogenesis of migraine headaches . Monoclonal anti-calcitonin gene-related peptide antibody can be used in immunoneutralization and radioimmunoassay. Mouse anti-calcitonin gene-related peptide antibody reacts specifically with an epitope present within the C-terminal ten amino acids of rat α-CGRP and also has shown cross reactivity with α and β-CGRP of humans and dogs. |
| Immunogen: | rat α-CGRP peptide. The epitope recognized by the antibody resides within the C-terminal ten amino acids of rat α-CGRP. |
| Packaging: | 100 μL in glass insert |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |