Anti-Cullin-1 antibody produced in rabbit
SIGMA/C7117 - affinity isolated antibody, buffered aqueous solution
Synonym: Anti-Cul1
MDL Number: MFCD02096156
Product Type: Chemical
Whole cell lysates were separated on SDS-PAGE and blotted with Anti-Cullin-1 (Cat. No. C7117). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and chemiluminescent substrate. Antibody dilution 1:1,000 Lanes 1. HEK293 cells (whole cell extract w/sample buffer) 2. HEK293 cells (RIPA lysate) 3. NIH3T3 cells (RIPA lysate) 4. Raji cells (RIPA lysate) 5, 6. HCT116 cells (RIPA lysate) 6. Inhibition of antibody binding with Cullin-1 immunizing peptide (human 759-776) (20 μg/mL)
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 85 kDa |
| shipped in | dry ice |
| species reactivity | human, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | western blot: 0.5-1 μg/mL using whole cell extract of human colon carcinoma HCT-116 cell line and whole cell extract of mouse fibroblasts NIH3T3 cell line |
| UniProt accession no. | Q13616   |
| Application: | Anti-Cullin-1 antibody produced in rabbit has been used in western blotting. |
| Biochem/physiol Actions: | Cullin 1(CUL1) is a scaffold protein of the ubiquitin E3 ligase Skp1/Cullin1/Rbx1/F-box protein complex, which ubiquitinates a broad range of proteins that are involved in cell-cycle progression, signal transduction and transcription. Cul1 plays an important role in protein degradation and protein ubiquitination. It regulates breast cancer cell proliferation, migration and invasion. Overexpression of Cul1 leads to breast cancer progression. CUL1 promotes human trophoblast cell invasion and dysregulation of its expression may be associated with pre-eclampsia (PE). It may have some role in rheumatoid arthritis (RA) and its response to methotrexate. Cullin1 may act as an important marker for human gastric cancer lymph node metastasis and prognosis. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Cullin-1 (Cul1) is encoded by the gene mapped to human chromosome 7q36.1. It is a 85 kDa protein that belongs to the family of cullin protein family containing distinct group of mediators (at least eight cullin members Cul1-8). |
| Immunogen: | synthetic peptide located at the C-terminus of human cullin-1 (amino acids 759-776). This sequence is identical in mouse cullin-1 and highly conserved (83% identity) in Drosophila cullin-1. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |