Monoclonal Anti-β-Catenin antibody produced in mouse
SIGMA/C7207 - clone 15B8, ascites fluid
Synonym: Monoclonal Anti-β-Catenin
MDL Number: MFCD00282178
Product Type: Chemical
Immunoblotting Madin Darby Bovine Kidney (MDBK) was separated on SDS-PAGE and probed with Monoclonal AB TO β CATENIN (MO) CL:15B8 (Cat. No. C7207). The antibody was developed with Alk Phos APA Mouse Fab ads HIgG (Cat. No. A1293) and a NBT/BCIP substrate. Antibody dilution 1/1,000.
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| application(s) | research pathology |
| biological source | mouse |
| clone | 15B8, monoclonal |
| conjugate | unconjugated |
| isotype | IgG1 |
| mol wt | antigen 94 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | chicken, bovine, human, canine |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: suitable |
| immunohistochemistry (frozen sections): suitable | |
| indirect immunofluorescence: 1:1000 using cultured MDBK cells | |
| microarray: suitable | |
| western blot: 1:1000 using cultured MDBK cells extract | |
| UniProt accession no. | P35222  |
| Application: | Monoclonal Anti-β-Catenin antibody produced in mouse has been used: • immunoblotting • immunohistochemistry • immunolabeling • immunofluorescence • immunocytochemical staining |
| Biochem/physiol Actions: | β-catenin is a prime component of the wingless (Wnt) pathway and plays a key role in gene expression during development. The homology between β-catenin and armadillo raised the possibility that β-catenin has a developmental signaling role in vertebrates. β-catenin mediates the interaction of the E-cadherin-catenin complex with the epidermal growth factor (EGF) receptor. β-catenin and plakoglobin are substrates for tyrosine phosphorylation following EGF stimulation of cells. β-catenin also associates with the tumor suppressor protein adenomatous polyposis coli (APC). Monoclonal antibody reacting specifically with β-catenin is an essential tool in defining the interactions and distributions of β-catenin and its relationships with other catenins and cadherins in various cells and tissues. |
| Biochem/physiol Actions: | Recognizes specifically mammalian and avian β-catenin. Does not cross-react with plakoglobin (γ-catenin). |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-β-Catenin (mouse IgG1 isotype) is derived from the 15B8 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. β-catenin belongs to the armadillo family and comprises the N-terminal region with 12 imperfect repeats and a conserved C-terminal region. The locus 3p22.1 in the human chromosome harbors β-catenin gene. β-catenin shares 70% sequence identity to a protein encoded by Drosophila armadillo, a segment polarity gene. Both armadillo and β-catenin share considerable homology with plakoglobin, which has been proposed to be γ-catenin. |
| Immunogen: | recombinant chicken β-catenin. |
| Physical form: | The product is provided as ascites fluid with 0.1% sodium azide as a preservative. |
| Preparation Note: | For extended storage freeze in working aliquots. For continuous use, store at 2-8 °C for up to one month. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |