Chymostatin
SIGMA/C7268 - microbial
Synonym: N-(Nα-Carbonyl-Cpd-X-Phe-al)-Phe (Cpd = capreomycidine) (capreomycidine = [S,S]-α-(2-Iminohexahydro-4-pyrimidyl)glycine)
CAS Number: 9076-44-2
MDL Number: MFCD00071059
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: C7268-100MG
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: C7268-1MG
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: C7268-25MG
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: C7268-50MG
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: C7268-5MG
| biological source | microbial |
| form | powder |
| InChI key | MRXDGVXSWIXTQL-HYHFHBMOSA |
| Quality Level | 300  |
| SMILES string | OC(C(NC(NC(C1NC(NCC1)=N)C |
| solubility | DMSO: 10 mM (Stock solutions stable for months at −20 °C.) |
| storage temp. | −20°C |
| Application: | Chymostatin has been used in a study that determined that molecular calculations are useful for evaluating the interactions between ligands, including inhibitors and homologous enzymes, in docking models. Chymostatin has also been used in a study to investigate the norovirus protease as an attractive target for antiviral drug development. |
| Biochem/physiol Actions: | Chymostatin is a strong inhibitor of many proteases, including chymotrypsin, papain, chymotrypsin-like serine proteinases, chymases, and lysosomal cysteine proteinases such as cathepsins A,B,C, B, H, and L. It weakly inhibits human leucocyte elastase. It is effective at a final concentration of 100 to 200 μg/ml (10 to 100 μM). Chymostatin is often included in protease inhibitor cocktails used with plant extracts. |
| Biochem/physiol Actions: | Inhibits the lysosomal proteinase cathepsin B, and the soluble Ca2+-activated proteinase. Many young plant tissues express a chomostatin-sensitive serine protease. |
| Other Notes: | A mixture of A (major), B and C components. A: X=Leu; B: X=Val; C: X=Ile chymostatin A MW = 607.7 chymostatin B MW = 593.7 chymostatin C MW = 607.7 |
| Packaging: | 1, 5, 25, 50 mg in glass bottle |
| Packaging: | 100 mg in poly bottle |
| Preparation Note: | Solubility testing in glacial acetic acid at 10 mg/ml yields a clear solution, which is usually colorless, but can be yellow in appearance. It is reportedly also soluble in DMSO; only slightly soluble in water and short-chain alcohols; insoluble in ethyl acetate, butyl acetate, ether, hexane, and petroleum ether. Stock solutions (10 mM) can be prepared in DMSO and are stable for months at -20 °C. Stock solutions can also be made in 0.1 M HCl. Dilute solutions (10-100 μM) are only stable for several hours, due to oxidation of the terminal aldehyde. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |