Anti-Calbindin-D-28K (KD-15) antibody produced in rabbit
SIGMA/C7354 - affinity isolated antibody, buffered aqueous solution
Synonym: Anti-CALB; Anti-D-28K
MDL Number: MFCD00164553
Product Type: Chemical
Enhanced Validation-By Independent Antibodies Formalin fixed, paraffin-embedded, methacarn-treated sections of Rat Cerebellum were stained with 10 μg/mL Anti-Calbindin-D-28K antibody, Mouse monoclonal, Clone: CB-955 (Cat. No. SAB4200543) (A) and developed using 20 μg/mL Anti-Mouse IgG (whole molecule)-Biotin antibody produced in Goat (Cat. No. B0529) and by 20 μg/mL ExtrAvidin®-Peroxidase (Cat. No. E2886), and 1:500 Anti-Calbindin-D-28K (KD-15) antibody produced in Rabbit (Cat. No. C7354) (B). The antibody was developed using using 20 μg/mL Anti-Rabbit IgG (whole molecule)-Biotin antibody produced in Goat (Cat. No. B8895) and by 20 μg/mL ExtrAvidin®-Peroxidase (Cat. No. E2886). Results Two Anti-Calbindin-D-28K antibodies,SAB4200543 (A) and C7354 (B) target different regions of Calbindin-D-28K show similar staining profiles between the two antibodies, demonstrating Independent Antibody Verification.
Immunohistochemistry Formalin fixed, paraffin-embedded Rat cerebellum were stained with 1:500 Anti-Calbindin-D-28K (KD-15) antibody produced in Rabbit (Cat. No. C7354) followed by 20 μg/mL Anti Rabbit IgG (WM)-Biotin (Cat. No. B8895) followed by 20 μg/mL ExtrAvidin®-Peroxidase (Cat. No. E2886).
Western Blotting Recombinant Rat calbindin-D-28K were separated on SDS-PAGE and probed with Anti-Calbindin-D-28K (KD-15) antibody produced in Rabbit (Cat. No. C7354) followed by 1:5,000 Anti-Rabbit IgG (whole molecule)-Alkaline Phosphatase antibody produced in Goat (Cat. No. A9919). Lanes 1. 1:1,000 antibody 2. 1:2,000 antibody 3. Negative Control
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| enhanced validation | independent Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous solution |
| mol wt | antigen 28 kDa |
| Quality Level | 200 |
| shipped in | dry ice |
| species reactivity | rat, chicken, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:500 using sections of rat, human or chicken cerebellum sections |
| microarray: suitable | |
| western blot: 1:1,000 using recombinant rat calbindin-D-28K | |
| UniProt accession no. | P05937  |
| Analysis Note: | Enzymatic predigestion of formalin-fixed, paraffin-embedded sections by proteolytic enzymes (e.g., 0.1% trypsin or protease, 10 min. at RT or 37 °C) improves immunohistochemical staining. |
| Application: | Anti-Calbindin- • immunocytochemistry • western blotting • microarray • immunohistochemistry |
| Biochem/physiol Actions: | Calbindin 1 (CALB) is a calcium-binding protein. The protein may play a role in development of Purkinje cells found in heterotopias and cerebellar dysgenesias. It also acts as a very important component of intracellular homeostasis in cerebellar neurons. The progressive decrease of calbindin content in the Purkinje cells undergoes degeneration and death during paraneoplastic changes in the cerebellum. Calbindin effects on Parkinson′s disease (PD) risk displays population specificity. |
| Biochem/physiol Actions: | Calbindin- |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Calbindin- |
| Immunogen: | synthetic peptide corresponding to the C-terminal region of rat calbindin-D-28K (amino acids 185-199). This sequence is identical in the corresponding human, mouse and bovine calbindin-D-28K sequences and is highly conserved (single amino acid substitution) in chicken and frog calbindin-D-28K. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide. |
| Hazard statements | H412 |
| Precautionary statements | P273 |
| Hazard Codes | Xn |
| Risk Statements | 21/22-52/53 |
| Safety Statements | 36/37-61 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |