Monoclonal Anti-Cyclin D1 antibody produced in mouse
SIGMA/C7464 - clone DCS-6, ascites fluid
MDL Number: MFCD00239664
Product Type: Chemical
Western Blotting JOURNAL CITATION: Wnt pathway activation in long term remnant rat model. By: Banon-Maneus, E., Rovira, J., et al. in Biomed Res Int, 2014. PubMed ID: 24995284 Image collected and cropped by CiteAb from the following publication, (http://www.hindawi.com/journals/bmri/2014/324713/), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunoblotting Human uterine mesodermal tumor cell extract was separated on SDS-PAGE and probed with Monoclonal AB to Cyclin D1 (DCS-6) (Cat. No. C7464). The antibody was developed with Anti-Mouse IgG (Fab specific), Alkaline Phosphatase conjugate (Cat. No. A1293) and a NBT/BCIP substrate. Lanes 1. Antibody diluted 1/200 2. Antibody diluted 1/400
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | DCS-6, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| isotype | IgG2a |
| mol wt | antigen 36 kDa (several lower and higher molecular weight bands) |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | rat, mouse, human, monkey |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: suitable |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable | |
| immunohistochemistry (frozen sections): suitable | |
| immunoprecipitation (IP): suitable | |
| microarray: suitable | |
| western blot: 1:200 using a human tumor cell line extract | |
| UniProt accession no. | P24385  |
| Application: | Monoclonal Anti-Cyclin D1 antibody produced in mouse may be used in: • immunoblotting • immunofluorescence • immunocytochemistry • immunoprecipitation • immunohistochemistry • antibody-mediated knock-out experiments |
| Biochem/physiol Actions: | Cyclin D1 is involved in regulating cyclin-dependent kinase (CDK) family members. Cyclin D1 and D2 are affiliated with CDK4 and/or CDK6 kinase activities that are observed in mid-G1 and culminate in the late G1 phase. Amplification of the cyclin D1 gene is observed in a significant percentage of cancers, including breast, bladder carcinoma, mantle cell lymphomas, head and neck cancers. This gene is also amplified in esophageal squamous cell carcinomas. |
| Biochem/physiol Actions: | Does not cross-react with other D-type cyclins. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-Cyclin D1 (mouse IgG2a isotype) is derived from the DCS-6 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Cyclin D1 protein is a G1-phase cyclin in mammalian cells. This protein is a D-type cyclin. The CCD1 gene is located on the human chromosome at 11q13.3. |
| Immunogen: | recombinant human Cyclin D1 protein. |
| Physical form: | The product is provided as ascites fluid with 15 mM sodium azide as a preservative. |
| Preparation Note: | For continuous use, store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |