Anti-Calretinin antibody produced in rabbit
SIGMA/C7479 - affinity isolated antibody, buffered aqueous solution
Synonym: Anti-CAB29; Anti-CAL2; Anti-CR
MDL Number: MFCD01321794
Product Type: Chemical
Immunohistochemistry Formalin fixed, paraffin-embedded Rat cerebellum tissue sections were stained with 1:2,000 Anti-Calretinin antibody produced in Rabbit (Cat. No. C7479) followed by 20 μg/mL Anti-Rabbit IgG (whole molecule)-Biotin antibody produced in Goat (Cat. No. B8895) and 25 μg/mL ExtrAvidin®-Peroxidase (Cat. No. E2886).
Immunoblotting
Recombinant Human Calretinin (6-HIS-CR) was separated on SDS-PAGE and probed with Anti-Calretinin (Cat. No. C7479). The antibody was developed with Anti-Rabbit IgG, alkaline phosphatase (Cat. No. A9919) and a NBT/BCIP substrate.
Antibody dilution 1:2,000.
Recombinant Human Calretinin (6-HIS-CR) was separated on SDS-PAGE and probed with Anti-Calretinin (Cat. No. C7479). The antibody was developed with Anti-Rabbit IgG, alkaline phosphatase (Cat. No. A9919) and a NBT/BCIP substrate.
Antibody dilution 1:2,000.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 29 kDa |
| Quality Level | 200  |
| species reactivity | rat, human, chicken |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:1,000 using formalin-fixed, paraffin-embedded rat cerebellum sections |
| microarray: suitable | |
| western blot: 1:1,000 using recombinant human calretinin | |
| UniProt accession no. | P22676  |
| Application: | Anti-Calretinin antibody produced in rabbit has been used in immunohistochemistry and immunofluorescence. |
| Application: | Anti-Calretinin antibody produced in rabbit is suitable for the following applications: • immunohistochemistry • microarray • western blotting at a dilution of 1:1,000 using recombinant human calretinin |
| Biochem/physiol Actions: | The physiological function of calretinin is not known, but it seems to be related to Ca2+ buffering and diffusion. Calretinin-deficient mice show impaired long-term potentiation (LTP) in the dentate gyrus, impaired motor coordination and Purkinje cell excitability. A role for both calretinin and calbindin-D-28K in synaptic plasticity and in neuroprotection from excitotoxic insults has been postulated. By acting as a buffer or sensor of intracellular free Ca2+, calretinin promotes calcium homeostasis. Furthermore, several truncated forms of calretinin have been found in certain tumor cells, but are absent from normal cells, suggesting that calretinin may play a role in tumorigenesis. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Anti-Calretinin is developed in rabbit using a synthetic peptide corresponding to the C-terminal region of rat calretinin (amino acids 194-209) conjugated to keyhole limpet hemocyanin (KLH) as immunogen. Calretinin is an intracellular Ca2+-binding protein, with broad tissue distribution and is a member of the calmodulin superfamily. It is expressed in certain populations of neurons in the central and peripheral nervous systems. It is abundant in the cerebellum (granule cells), olfactory bulb and auditory pathways. |
| Immunogen: | synthetic peptide corresponding to the C-terminal region of rat calretinin (amino acids 194-209) conjugated to KLH. This sequence is specific for calretinin and not found in other members of the EF-hand family such as calbindin-D-28K, calbindin-D-9K, myosin light chain, parvalbumin, S-100a, S-100b, S100A2 (S100L) and S100A6 (calcyclin). This sequence is identical in human and mouse calretinin and highly conserved in chicken calretinin. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin, and 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |