Anti-CENP-E antibody produced in rabbit
SIGMA/C7488 - IgG fraction of antiserum, buffered aqueous solution
Synonym: Anti-
MDL Number: MFCD05665206
Product Type: Chemical
Immunoblot Whole cell extract of A431 cells treated with nocodazole was separated on SDS-PAGE and blotted with Rabbit Anti-CENP-E (Cat. No. C7488). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and a chemiluminescent substrate. Lanes A. Antibody dilution 1:2,000 B. Antibody dilution 1:2,000 + CENP-E immunizing peptide (10 μg/mL)
| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~310 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: suitable using human epitheloid carcinoma HeLa cell line. |
| microarray: suitable | |
| western blot: 1:2,000 using whole extract of HeLa nuclear cell line | |
| UniProt accession no. | Q02224  |
| Application: | Anti-CENP-E antibody produced in rabbit has been used in: • immunoblotting • immunofluorescence • immunocytochemistry. |
| Biochem/physiol Actions: | Centromere-associated protein-E (CENP-E) plays an important role in attachment of kinetochores to spindle microtubules in the alignment of chromosomes and is an essential component of mitotic checkpoint signaling cascade. It functions as a motor in the initial chromosome movement at the mitotic midzone. CENP-E is associated with minus end-directed microtubule motor activity suggesting that CENP-E might be responsible for poleward kinetochore movements in prometaphase and anaphase A. Suppression of CENP-E synthesis by antisense CENP-E yields chromosomes that are chronically mono-oriented with flattened bipolar spindles and generates spindle poles fragments. Depletion of CENP-E leads to profound checkpoint activation and long-term mitotic arrest. CENP-E has been implicated as a binding partner for the mitotic checkpoint kinase BubR1 during mitosis. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Centromere-associated protein-E (CENP-E, 312 kDa) is a member of the kinesin superfamily of microtubule motor proteins and is an integral part of kinetochore corona fibers that link centromere to the spindle microtubules. CENP-E localizes to the kinetochore throughout all phases of mitotic chromosome movement from early prometaphase through anaphase A. |
| Immunogen: | synthetic peptide corresponding to amino acids 2545-2563 located near the C-terminus of human CENP-E conjugated to KLH. |
| Packaging: | 200 μL in glass insert |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |