Butyrylcholinesterase from equine serum
SIGMA/C7512 - lyophilized powder, ≥10 units/mg protein
Synonym: Acylcholine acyl-hydrolase; Choline esterase, butyryl; Pseudocholinesterase
CAS Number: 9001-08-5
EC Number: 232-579-2
MDL Number: MFCD00081455
Product Type: Chemical
Common enzymatic methods for measuring organophosphate and cholinesterase activity include: colorimetric (using DTNB or ferricyanate to measure thiolesterase products), colorimetric (coupled with choline oxidase and peroxidase), fluorescent, titrimetric (mainly for purified enzyme activity assays). Shown above is the simple uncoupled assay using acetylcholine which can be measured titrimetrically.
Common enzymatic methods for measuring organophosphate and cholinesterase activity include: colorimetric (using DTNB or ferricyanate to measure thiolesterase products), colorimetric (coupled with choline oxidase and peroxidase), fluorescent, titrimetric (mainly for purified enzyme activity assays). Shown above is the coupled assay using choline oxidase and peroxidase.
Common enzymatic methods for measuring organophosphate and cholinesterase activity include: colorimetric (using DTNB or ferricyanate to measure thiolesterase products), colorimetric (coupled with choline oxidase and peroxidase), fluorescent, titrimetric (mainly for purified enzyme activity assays). Shown above is the thiolesterase assay using DTNB detection.
Common enzymatic methods for measuring organophosphate and cholinesterase activity include: colorimetric (using DTNB or ferricyanate to measure thiolesterase products), colorimetric (coupled with choline oxidase and peroxidase), fluorescent, titrimetric (mainly for purified enzyme activity assays). Shown above is the thiolesterase assay using ferricyanate detection.
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: C7512-1.2KU
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: C7512-12KU
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: C7512-6KU
| composition | Protein, ≥60% |
| form | lyophilized powder |
| mol wt | tetramer 440 kDa |
| Quality Level | 200  |
| solubility | cold water: 60 U/mL |
| specific activity | ≥10 units/mg protein |
| storage temp. | −20°C |
| Analysis Note: | Protein determined by biuret |
| Application: | Butyrylcholinesterase from equine serum has been used in a microcalorimetric study of the inhibition of butyrylcholinesterase by paraoxon. This enzyme has also been used in a study to investigate the synthesis and inhibition of cholinergic enzymes. Selective inhibition of BChE activity can be used in the detection of organophosphates. Its use in the treatment of organophosphate toxicity has shown clinical potential, as there is a correlation between the blood level of BChE in humans and the degree of protection against toxic nerve agents. There has also been an interest in the roles of cholinesterases with regard to Alzheimer’s disease. Investigations into selective inhibitors may provide a clearer picture of the physiological role of BChE in both healthy and diseased individuals. This product has been used for the screening of cholineesterase inhibitors in selected fruits and vegetables, for restoring cognitive function and improving memory. It has also been used to develop a butyrylcholinesterase and choline oxidase immobilized bio-sniffer for the detection of nicotine. Nicotine inhibits BChE activity. A decrease in the byproducts of BChE activity reflects the volume of nicotine. |
| Biochem/physiol Actions: | Butyrylcholinesterase (BChE) can, unlike AChE, efficiently hydrolyze larger esters of choline such as butyrylcholine and benzoylcholine. The enzyme is activated by Ca2+ and Mg2+ and the activity is constant over the pH range 6.0-8.0. It is inhibited by betaine, nicotine, organophosphates, and carbamates. |
| Biochem/physiol Actions: | Mutations in butyrylcholinesterase |
| General description: | Butyrylcholinesterase (BChE) is a serine hydrolase that is structurally similar to acetylcholinesterase (AChE), but differs in substrate specificities and inhibitor sensitivities. The enzyme is a tetrameric glycoprotein with four equal subunits (110 kDa each). It is also termed as pseudocholinesterase. It is produced in the liver. It is found in blood, the synapse of neuromuscular junctions and glia cells and axons of white matter. |
| Other Notes: | One unit will hydrolyze 1.0 μmole of butyrylcholine to choline and butyrate per min at pH 8.0 at 37 °C. The activity obtained using butyrylcholine as substrate is ~2.5 times that obtained using acetylcholine. |
| Packaging: | 1200, 6000, 12000 units in poly bottle |
| Physical form: | Lyophilized powder containing buffer salts |
| Preparation Note: | This product is prepared from equine serum using ammonium sulfate fractionation and supplied as a lyophilized powder. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥10 units/mg protein |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 3.1.1.8 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |