Collagen from human placenta
SIGMA/C7521 - Bornstein and Traub Type IV, powder
Synonym: Collagen Type 4
CAS Number: 9007-34-5
EC Number: 232-697-4
MDL Number: MFCD00130825
Product Type: Chemical
Collagen Type IV is a triple helix composed predominantly of two α1(IV) and one α2(IV), heterotrimers may be composed of six different α(IV)chains
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: C7521-5MG
| application(s) | diagnostic assay manufacturing |
| biological source | human placenta |
| description | Sigma Type VI |
| form | powder |
| impurities | HIV, hepatitis B and hepatitis C, none detected |
| Quality Level | 200  |
| solubility | 0.5 M acetic acid: 1 mg/mL |
| storage temp. | −20°C |
| suitability | suitable for substrate for collagenase |
| UniProt accession no. | P08572  |
| P29400 |
|
| P53420 |
|
| Q01955 |
|
| Q14031 |
| Analysis Note: | An SDS polyacrylamide gel electrophoresis test run under reducing conditions consistent with basement membrane collagen yields three major bands. |
| Biochem/physiol Actions: | During development, collagen IV is ubiquitously distributed in BMs. During the maturation process, this network gets partially replaced in a remarkably tissue specific manner, defining BM structure and function. Collagen IV has been shown to bind to platelets, hepatocytes, keratinocytes, endothelial, mesangial, pancreatic cells and some tumor cells. |
| Other Notes: | All collagen molecules are composed of three polypeptide chains arranged in a triple helical conformation, with a primary structure that is mostly a repeating motif with glycine in every third position and proline or 4-hydroxyproline frequently preceding the glycine residue. Type IV collagen occurs only in the basement membranes and contains up to six genetically distinct a-chains. |
| Other Notes: | Collagen is classified into a number of structurally and genetically distinct types. We use the nomenclature proposed by Bornstein and Traub. Be wary of confusing Sigma-type designations with recognized collagen classification types. |
| Packaging: | 10, 50 mg in poly bottle |
| Packaging: | 5 mg in glass bottle |
| Preparation Note: | This is a lyophilized powder that can be reconstituted in sterile .5 M acetic acid, PBS or water at 1 mg/mL. A PBS solution will be stable for at least one year at -20°C. It is prepared by a modification of the pepsin extraction method of Glanville, R.W., et al. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |