Chloroform
SIGMA/C7559 - ≥99%, PCR Reagent, contains amylenes as stabilizer
Synonym: Methylidyne trichloride; Trichloromethane
CAS Number: 67-66-3
Empirical Formula (Hill Notation): CHCl3
Molecular Weight: 119.38
EC Number: 200-663-8
MDL Number: MFCD00000826
Linear Formula: CHCl3
Product Type: Chemical
| assay | ≥99% |
| bp | 60.5-61.5 °C (lit.) |
| color | colorless |
| contains | amylenes as stabilizer |
| density | 1.492 g/mL at 25 °C (lit.) |
| foreign activity | DNase, RNase, none detected |
| form | liquid |
| InChI | 1S/CHCl3/c2-1(3)4/h1H |
| InChI key | HEDRZPFGACZZDS-UHFFFAOYSA |
| mp | −63 °C (lit.) |
| packaging | vial of 1.5 mL |
| Quality Level | 200  |
| refractive index | n |
| SMILES string | ClC(Cl)Cl |
| technique(s) | PCR: suitable |
| vapor density | 4.1 (vs air) |
| vapor pressure | 160 mmHg ( 20 °C) |
| Application: | Chloroform has been used in phenol-chloroform extraction to find its ability to purify nucleic acids from blood culture fluids. It has also been used in RNA extraction/isolation and purification. |
| General description: | Chloroform is used in pharmaceutical preparations as an anesthetic and as an additive. It is now mainly used as an organic solvent. Chloroform is produced by anthropogenic and natural sources. This abundant halocarbon in the atmosphere is found in ground and surface water sources. |
| Packaging: | 5 vials in serum bottle |
| Symbol |   GHS06,GHS08 |
| Signal word | Danger |
| Hazard statements | H302 - H315 - H319 - H331 - H336 - H351 - H361d - H372 - H412 |
| Precautionary statements | P201 - P273 - P301 + P312 + P330 - P302 + P352 - P304 + P340 + P311 - P308 + P313 |
| Hazard Codes | Xn |
| Risk Statements | 63-20-22-36/38-40-48/20/22-67 |
| Safety Statements | 26-36/37 |
| RIDADR | UN 1888 6.1 / PGIII |
| WGK Germany | WGK 3 |
| Flash Point(F) | does not flash |
| Flash Point(C) | does not flash |
| Purity | ≥99% |
| bp | 60.5-61.5 °C (lit.) |
| mp | −63 °C (lit.) |
| Density | 1.492 g/mL at 25 °C (lit.) |
| Refractive Index | n |
| UNSPSC | 12191502 |