Casein Blocking Buffer
SIGMA/C7594 - for Northern and Southern blotting, powder blend
Synonym: blocking buffer
EC Number: 232-555-1
Product Type: Chemical
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| form | powder blend |
| grade | Molecular Biology |
| pH | 6.8-7.2(reconstituted per directions) |
| Quality Level | 200  |
| solubility | H2O: soluble, faintly yellow (dissolves in 5-10 minutes.) |
| storage temp. | room temp |
| technique(s) | Southern blotting: suitable |
| Application: | Suitable as a blocking Buffer for Western, Southern and Northern Blot membranes. Blocked membranes can then be used in either chemiluminescent, colorimetric, or DIG detection assays. |
| Features and Benefits: | • Better blocking performance than dried milk or BSA • Compatible with neutral and positively-charged membranes • Compatible with chemiluminescent, colorimetric , and DIG detection assays |
| General description: | Casein is a milk-derived substance routinely used in blocking buffers for Western, Southern and Northern Blot procedures. Blocking agents are used to reduce non-specific binding of detection reagents to the membranes. |
| Packaging: | 1 L in poly bottle |
| Preparation Note: | Dissolve entire contents of bottle in 800 mL molecular biology grade water. Stir until well mixed, then add water to final volume of 1L. Store at 2-8°C for up to one week. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Storage Temp. | room temp |
| UNSPSC | 12161700 |