Anti-Calnexin antibody, Mouse monoclonal
SIGMA/C7617 - clone TO-5, purified from hybridoma cell culture
Synonym: Anti-CANX; Anti-CNX
MDL Number: MFCD01094399
Product Type: Chemical
Enhanced Validation-By Independent Antibodies Immunofluoresence. HeLa cells were fixed and permeabilized with methanol followed by acetone. Fixed cells were stained with 1:200 Anti-Calnexin antibody produced in Rabbit (Cat. No. C4731) (A). The antibody developed using Goat Anti-Rabbit IgG, FITC Conjugate antibody, and 1:400 Anti-Calnexin antibody, Mouse Monoclonal, Clone: TO-5 (Cat. No. C7617) (B). The antibody was developed using Goat Anti-Mouse IgG, Cy3 Conjugate antibody. Results Two Anti Calnexin antibodies, C4731 (A) and C7617 (B) target different regions of Calnexin show similar staining profiles between the two antibodies, demonstrating Independent Antibody Verification.
Immunoblotting HeLa cell extract was separated on SDS-PAGE and probed with Monoclonal PA TO CALNEXIN (MO) CL:TO-5 (Cat. No. C7617). The antibody was developed with HRP APA Mouse Fab ads/HuBovHor (gt) (Cat. No. A2304) and an ECL substrate. Antibody concentration 1μg/mL.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | TO-5, monoclonal |
| concentration | ~2 mg/mL |
| conjugate | unconjugated |
| enhanced validation | independent Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen ~90 kDa |
| Quality Level | 200 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | flow cytometry: suitable |
| immunocytochemistry: suitable | |
| immunohistochemistry: suitable | |
| indirect ELISA: suitable | |
| western blot: 0.5-1.0 μg/mL using total cell extract of HeLa cells. | |
| UniProt accession no. | P27824  |
| Application: | Anti-Calnexin antibody, Mouse monoclonal has been used in: • enzyme-linked immunosorbent assay (ELISA) • immunoblotting • flow cytometry • immunocytochemistry • immunohistochemistry • immunofluorescence analysis |
| Biochem/physiol Actions: | Mouse monoclonal clone TO-5 anti-Calnexin antibody recognizes human Calnexin, ~90 kDa. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Anti-Calnexin antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hybridoma TO-5 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. Calnexin contains a long (461 amino acids) N-terminal Ca2+-binding domain extending into the lumen of the endoplasmic reticulum (ER), a short (22 amino acids) trans-membrane segment and an acidic cytosolic domain (96 amino acids). |
| General description: | Calnexin (p88, IP90) is a calcium-binding, type I integral membrane protein, localized primarily in the endoplasmic reticulum (ER). Calnexin binds newly synthesized glycoproteins and misfolded proteins and is believed to play a critical role in quality control processes during protein synthesis and folding. Calnexin acts as a lectin like chaperone that binds oligosaccharide residues of newly synthesized N-linked glycoproteins. The lectin specificity of calnexin and its soluble homologue calreticulin has been identified as high mannose oligosaccharides terminating in monoglucosyl residues linked through α1-3. Calnexin has been shown to be associated with several cell surface proteins, including MHC class I heavy chain, T-cell receptor (TCR), and B cell membrane immunoglobulin during translocation through the ER. It also forms complexes with other resident ER proteins involved in Ca2+-dependent retention of proteins. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |