Chaperonin 60 from Escherichia coli
SIGMA/C7688 - >95% (SDS-PAGE), recombinant, expressed in E. coli overproducing strain, lyophilized powder
Synonym: Cpn60; GroEL
MDL Number: MFCD00163256
Product Type: Chemical
SDS-PAGE Purity of Chaperonin 60 from Escherichia coli(Cat. No. C7688) was assessed on SDS-PAGE.
Folding activity of Gro EL (Chaperonin 60) Testing the folding activity of Gro EL system (Chaperonin 60 from Escherichia coli, Cat. No. C7688) and the refolding of Rhodanese by Gro EL. The Chaperonin protein Gro EL (Chaperonin 60) is a heat shock protein that assist the folding of nascent and heat destabilized proteins. In vitro Gro EL promote protein refolding and reactivation. Reconstitution of denatured enzymes such as the photosynthetic enzyme Rubisco1, mitochondrial Rhodanese2 and Glutamine synthase3 were reported. S1: Denaturated Rhodanese + Chaperonin 60 S2: Native Rhodanese + Chaperonin 60 S3: Denaturated Rhodanese S4: Native Rhodanese
| assay | >95% (SDS-PAGE) |
| biological source | Escherichia coli |
| form | lyophilized powder |
| Quality Level | 200  |
| recombinant | expressed in E. coli overproducing strain |
| storage temp. | 2-8°C |
| suitability | passes test (Refolding (w/GroES)) |
| technique(s) | electron microscopy: suitable |
| mass spectrometry (MS): suitable | |
| UniProt accession no. | P10809  |
| Application: | Chaperonin 60 from Escherichia coli has been used: • in mass spectroscopy • in cryo-electron microscopy imaging as control for testing particle distribution • as standard in infrared spectrum measurements • as a protein sample for stability assessment and characterization studies using differential mobility analysis (DMA) and cryo-electron microscopy (cryo-EM). |
| Biochem/physiol Actions: | Apart from its role in facilitating protein folding, chaperonin 60 (Cpn60) serves as an extracellular signaling protein, showing functional similarities to certain proinflammatory cytokines. Furthermore, Cpn60 contributes to the inhibition of lipid accumulation and adipogenesis during the initial stages of cellular differentiation, thereby exerting anti-obesity effects. |
| General description: | Research area: Cell Signaling Chaperonin 60 (GroEL) and chaperonin 10 (GroES) belong to the ubiquitous family of heat-shock molecular chaperones found in prokaryotes and in eukaryotic organelles. |
| Packaging: | 1 mg in serum bottle |
| Packaging: | Package size based on protein content. |
| Physical form: | Lyophilized powder containing Tris buffer salts, potassium chloride, magnesium chloride, dithiothreitol, and trehalose as stabilizer. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | >95% (SDS-PAGE) |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352200 |