Cycloheximide

SIGMA/C7698 - from microbial, ≥94% (TLC)

Synonym: 3-[2-(3,5-Dimethyl-2-oxocyclohexyl)-2-hydroxyethyl]glutarimide; Actidione; Naramycin A

CAS Number: 66-81-9
Empirical Formula (Hill Notation): C₁₅H₂₃NO₄
Molecular Weight: 281.35
EC Number: 200-636-0
MDL Number: MFCD00082346
Linear Formula: C₁₅H₂₃NO₄
Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-C7698-1G	1 g	$85.50 1/EA	Add To Favorites
45-C7698-5G	5 g	$232.00 1/EA	Add To Favorites

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This image depicts SKU: C7698-1G

Properties
Descriptions
Safety Info.
Basic Data

antibiotic activity spectrum	fungi
	yeast
assay	≥94% (TLC)
biological source	microbial
color	white to off-white
form	powder
InChI	1S/C15H23NO4/c1-8-3-9(2)15(20)11(4-8)12(17)5-10-6-13(18)16-14(19)7-10/h8-12,17H,3-7H2,1-2H3,(H,16,18,19)/t8-,9-,11-,12+/m0/s1
InChI key	YPHMISFOHDHNIV-FSZOTQKASA-N
mode of action	protein synthesis \| interferes
Quality Level	200
SMILES string	[H][C@]1(C[C@@H](C)C[C@H](C)C1=O)[C@H](O)CC2CC(=O)NC(=O)C2
solubility	ethanol: soluble, clear to hazy
storage temp.	2-8°C

Application:	• In yeast strains, cycloheximide has been used as a protein synthesis inhibitor in the cycloheximide chase experiment. • It has been used to inhibit translation in mammalian cells. • It has been used to suppress fungal growth.
Biochem/physiol Actions:	Mode of Action: Translation inhibition in eukaryotes resulting in cell growth arrest and cell death. CHX is widely used for the selection of CHX-resistant strains of yeast and fungi, controlled inhibition of protein synthesis for detection of short-lived proteins and super-induction of protein expression, and apoptosis induction or facilitation of apoptosis induction by death receptors. Activity Spectrum: Active against yeast and fungi like Candida, Aspergillus, Saccharomyces, Penicillium
Features and Benefits:	• High-quality antibiotic suitable for multiple research applications • Commonly used in Cell Biology and Biochemical applications
General description:	Cycloheximide, also known as Actidione, is a glutarimide antibiotic commonly derived from the bacterium Streptomyces griseus. It acts as a potent inhibitor of protein biosynthesis in eukaryotic cells by disrupting the translocation step during translation, effectively blocking translational elongation. In microbiology, it plays a crucial role as a selection agent for resistant strains of yeast and fungi, proving invaluable in controlled experimental environments. In cell biology and biochemical research, Cycloheximide showcases a dual nature concerning Apoptosis Induction, inducing or inhibiting apoptosis depending on the cell type. Its rapid and reversible effects make it an ideal choice for studying cellular processes and determining protein half-life. Cycloheximide also finds extensive applications in biomedical research, where it inhibits protein synthesis in eukaryotic cells studied in vitro (outside of organisms) and its effects are rapidly reversed by simply removing it. This makes Cycloheximide a go-to choice for exploring cell biology, biomedical and biochemical research, offering precise control and versatility in experiments.
Other Notes:	For additional information on our range of Biochemicals , please complete this form .
Other Notes:	Keep container tightly closed in a dry and well-ventilated place. Keep in a dry place.
Packaging:	1, 5 g in poly bottle
Preparation Note:	Tightly closed. Dry. Keep in a well-ventilated place. Keep locked up or in an area accessible only to qualified or authorized persons.

Symbol	GHS06,GHS08,GHS09
Signal word	Danger
Hazard statements	H300 - H341 - H360D - H411
Precautionary statements	P201 - P202 - P264 - P270 - P273 - P301 + P310
Hazard Codes	T+,N
Risk Statements	61-28-51/53-68
Safety Statements	53-45-61
RIDADR	UN2811 - class 6.1 - PG 2 - EHS - Toxic solids, organic, n.o.s.,
WGK Germany	WGK 3
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Purity	≥94% (TLC)
Storage Temp.	2-8°C
UNSPSC	12352111

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