Monoclonal Anti-VSV Glycoprotein−Cy3™ antibody produced in mouse
SIGMA/C7706 - clone P5D4, purified immunoglobulin, buffered aqueous solution
Synonym: Monoclonal Anti-VSV Glycoprotein
Product Type: Chemical
Immunofluorescence 293-T cells expressing Vinculin tagged at C-terminus with VSV-G were fixed and permeabilized with 4% paraformaldehyde followed by 0.05% Triton X-100. Fixed cells were stained with 1:10,000 Monoclonal Anti-VSV Glycoprotein-Cy3™ antibody produced in Mouse, Clone: P5D4 (Cat. No. C7706).
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | P5D4, monoclonal |
| conjugate | CY3 conjugate |
| form | buffered aqueous solution |
| isotype | IgG1 |
| Quality Level | 200  |
| shipped in | wet ice |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | direct immunofluorescence: 1:10,000 using COS-7 cells transfected with a VSV-G tagged vinculin construct |
| Application: | A dilution of 1:1000 of Monoclonal Anti-VSV Glycoprotein-Cy3™ antibody produced in mouse was used to detect VSV glycoprotein in pig sperms by immunofluorescence. |
| Application: | Monoclonal Anti-VSV Glycoprotein Cy3™ antibody produced in mouse has been used: • in studies applying • microinjection of antibody • immunoblotting • immunoprecipitation • immunocytochemistry • immunoelectron microscopy. |
| Biochem/physiol Actions: | The antibody recognizes an epitope containing the five carboxy-terminal amino acids of VSV Glycoprotein. In infected cells, the antibody localizes the immature forms of VSV-G in the rough endoplasmic reticulum (RER) and in the cisternae of Golgi complex, as well as mature VSV-G at the cell surface and in the budding virus. The antibody does not stain the secreted form of VSV-G which lacks the membrane and the cytoplasmic domain. This antibody has been used for studies on the role of the cytoplasmic domain on newly-synthesized VSV-G during transfer to the plasma membrane and cell surface, using micro-injected antibody, immunoblotting, immunoprecipitation, immunocytochemistry and immunoelectron microscopy. The antibody has been used for the detection, immunoprecipitation and immunocytochemical staining of exogenously introduced constructs tagged with the carboxyl-terminus of VSV-G. This tag does not interfere with the function of the studied protein and can be specifically recognized by the P5D4 antibody without cross-reaction with any endogenous protein. |
| Biochem/physiol Actions: | The envelope of vesicular stomatitis virus (VSV) consists of a bilayer membrane with a single type of glycoprotein, the G-protein (VSV-G) which mediates attachment to the cell surface and induces pH-dependent fusion between viral and target membranes. The carboxyl terminus of the VSV-G protein which does not have any homology with cellular proteins, has been engineered into expression vectors as a tag. Proteins expressed with this tag may thus be detected and localized using an antibody reactive specifically against this epitope with no risk of cellular background staining. |
| Biochem/physiol Actions: | The vesicular stomatitis virus (VSV) glycoprotein facilitates the entry of HIV into the CD4 T cells through endocytotic pathway. The coupling of Cy3 to Anti-VSV Glycoprotein antibody allows for the visualization of protein by fluorescent microscopy. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-VSV Glycoprotein (mouse IgG1 isotype) is derived from the P5D4 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with a synthetic peptide of vesicular stomatitis virus glycoprotein (VSV-G), conjugated to keyhole limpet hemocyanin (KLH). VSV-G is a protein expressed on the surface of bullet shaped virions. |
| Immunogen: | synthetic peptide containing the 15 carboxy-terminal amino acids (497-511) of Vesicular Stomatitis Virus Glycoprotein (VSV-G), conjugated to KLH. |
| Legal Information: | Cy is distributed under license from Amersham Biosciences Limited. |
| Legal Information: | Cy3 is a trademark of Cytiva |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |