Anti-Caspase 9 antibody produced in rabbit
SIGMA/C7729 - affinity isolated antibody, buffered aqueous solution
Synonym: Anti-APAF-3; Anti-APAF3; Anti-ICE-LAP6; Anti-MCH6; Anti-PPP1R56
MDL Number: MFCD01864176
Product Type: Chemical
Immunohistochemistry Formalin fixed, paraffin-embedded Rat Heart tissue sections were stained with 20 μg/mL Anti-Caspase 9 antibody produced in Rabbit (Cat. No. C7729) followed by 20 μg/mL Anti-Rabbit IgG (whole molecule)-Biotin antibody produced in Goat (Cat. No. B8895) and by 20 μg/mL ExtrAvidin®-Peroxidase (Cat. No. E2886).
Western Blotting Whole extract of Jurket cells were separated on SDS-PAGE and probed with Anti-Caspase 9 antibody produced in Rabbit (Cat. No. C7729). The antibody was developed using 1:5,000 Anti-Rabbit IgG (whole molecule)-Peroxidase antibody produced in Goat (Cat. No. A0545). Lanes 1. 1:300 antibody 2. 1:600 antibody 3. Negative Control
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 35-47 kDa (two bands) |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:40 using microwave-treated tissue sections of human and rat heart |
| immunoprecipitation (IP): 10 μg using HeLa mitochondrial RIPA lysate (500 μg) | |
| microarray: suitable | |
| western blot: 1:300 using whole extract of human Jurkat cells | |
| UniProt accession no. | P55211  |
| Application: | Anti-Caspase 9 antibody produced in rabbit has been used in western blot analysis. |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Western Blotting (1 paper) |
| Biochem/physiol Actions: | Caspase 9/CASP9 is essential for apoptosis during normal development of the murine central nervous system and plays a role in controlling tumor development. It functions as an initiator caspase when mitochondrial dysfunction is the primary event in apoptosis. Caspase 9 functions as an initiator caspase when mitochondrial dysfunction is the primary event in apoptosis. Caspase 9 can also be activated by granzyme B. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Anti-Caspase 9 is developed in rabbit using a synthetic peptide corresponding to amino acid of human procaspase 9 with N-terminal added lysine conjugated to keyhole limpet hemocyanin (KLH) with glutaraldehyde. Caspase 9/CASP9 (Mch6, ICE-LAP6, APAF3) is a member of the caspase 2 subfamily and is classified together with caspases 8 and 10 as an initiator caspase. It is a cysteine containing aspartate specific protease, made up of 416 amino acids, of which 13 are cysteine. CASP9 is located on human chromosome 1p36.21. |
| Immunogen: | synthetic peptide corresponding to amino acid residues 299-316 of human procaspase 9 with N-terminal added lysine conjugated to KLH with glutaraldehyde. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |