Anti-Centrin antibody produced in rabbit
SIGMA/C7736 - affinity isolated antibody, buffered aqueous solution
Synonym: Anti-CEN1; Anti-CETN
MDL Number: MFCD03791471
Product Type: Chemical
Immunofluorescence NIH3T3 cells were fixed and stained with 6 μg/mL Anti-Centrin (Cat. No. C7736). The antibody was developed using Goat Anti-Rabbit IgG, Atto488 conjugate. Nuclear staining with DAPI.
Immunofluorescence HeLa cells were fixed and stained with 6 μg/mL Anti-Centrin (Cat. No. C7736). The antibody was developed using Goat Anti-Rabbit IgG, Atto488 conjugate. Nuclear staining with DAPI.
Immunoblotting Lysate of HEK-293T cells overexpressing human centrin-1 was separated on SDS-PAGE, blotted with Anti-Centrin (Cat. No. C7736) and developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. Antibody 0.3 μg/mL 2. Antibody 0.3 μg/mL + centrin1 immunizing peptide (human, 157-172)
Immunoblotting Lysates of HT-29 (lanes 1,2), HEK-293T (lanes (3-4) and HeLa (lanes 5-6) cells were separated on SDS-PAGE, blotted with 0.3 μg/mL Anti-Centrin (Cat. No. C7736) and developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1,3,5. Antibody 0.3 μg/mL 2,4,6. Antibody 0.3 μg/mL + centrin1 immunizing peptide (human, 157-172)
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 21 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | microarray: suitable |
| western blot: 1:2,000 using 293-T exp.Hu.Centrin-1LY117608 or 293-T exp.Hu.Centrin1-FLAG LY401316 | |
| UniProt accession no. | Q12798  |
| Application: | Anti-Centrin antibody produced in rabbit has been used in: • immunofluorescence • immunoblotting • immunofluorescence microscopy |
| Biochem/physiol Actions: | Centrin has a fundamental role in the microtubule-organizing center (MTOC) structure and function. These proteins has imperative roles in centrosome positioning and segregation. Centrin also participates in cytokinesis and cell cycle progression. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Centrins are acidic proteins, that are usually found in all eukaryotes. Centrin 1 (CETN1), an intron-less gene is mapped on human chromosome 18p11. It possesses certain features of an expressed retroposon. |
| General description: | The centrosome, the major microtubule-organizing center (MTOC) in the animal cell, is a key determinant in the organization of the cytoplasm, in the determination of cell polarity, and in the establishment of mitotic spindle poles during the cell cycle. Centrin is a member of the calcium binding E-F hand protein superfamily present in the centrosome pericentriolar matrix. Three separate centrin genes have been identified in human cells: centrin 1, 2, and 3. Centrin 1 shows strong amino acid homology to centrin 2 (85%), but lower homology to centrin 3 (54%) and CDC31 (50%) from the yeast S. cerevisiae. Centrin has a fundamental role in the MTOC structure and function. Phenotypic analysis of mutants in Chlamydomonas caltractin and the yeast CDC31 genes have indicated that the protein is required for proper duplication and segregation of the MTOC in the respective cells. |
| Immunogen: | synthetic peptide corresponding to the C-terminus of human centrin 1 (amino acids 157-172). The sequence is highly conserved in centrin 1 of many species, e.g. mouse, Drosophila, and Xenopus. It is also found in human and mouse centrin 2, but is not found in centrin 3. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |