Anti-β-Catenin−Cy3 antibody, Mouse monoclonal
SIGMA/C7738 - clone 15B8, purified from hybridoma cell culture
Synonym: Monoclonal Anti-β-Catenin
MDL Number: MFCD00282178
Product Type: Chemical
Immunofluorescence MDBK Madin Darby Bovine Kidney cells were fixed and permeabilized with methanol followed by acetone. Fixed cells were stained with 1:100 Anti-β-Catenin-Cy3™ antibody, Mouse monoclonal, Clone: 15B8 (Cat. No. C7738). Cells were counterstained with DAPI (blue) to stain nuclei.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 15B8, monoclonal |
| concentration | ~1 mg/mL |
| conjugate | CY3 conjugate |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen 94 kDa |
| Quality Level | 200  |
| shipped in | wet ice |
| species reactivity | chicken, bovine, human, canine |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | direct immunofluorescence: 1:100 using methanol/acetone-fixed bovine MDBK/MCF-7 Cell Line |
| UniProt accession no. | P35222  |
| Application: | Anti-β-Catenin-Cy3 antibody has been used in • direct immunofluorescence • western blotting • staining • immunohistochemistry |
| Application: | Suitable for direct immunofluorescence at a dilution of 1:100 using methanol/acetone-fixed bovine MDBK/MCF-7 cells. The antiobody has been used for western blot analysis in mouse embryonic stem cells. |
| Biochem/physiol Actions: | Catenin (cadherin-associated protein) β 1 also called β-catenin is encoded by the CTNNB1 gene in humans. It is a dual function protein that regulates the coordination of cell-cell adhesion and gene transcription. It is useful for differential diagnosis of osteoblastoma (OB) and osteosarcoma (OS). β-catenin signaling pathway may contribute to cholangiocarcinoma (CCA) cell proliferation. It may serve as a prognostic marker for CCA progression and provide a potential target for CCA therapy. Mutations in β-catenin are associated with ovarian carcinoma. In addition, mutations in β-catenin are linked to intellectual disability. NEK2 (Never in mitosis A-related kinase 2) binds and phosphorylates β-catenin, thereby regulating centrosome seperation. Focal adhesion protein, Kindlin-2, enhances WNT signaling by forming a transcriptional complex with β-catenin. |
| Biochem/physiol Actions: | Recognizes specifically mammalian and avian β-catenin. Does not cross-react with plakoglobin (γ-catenin). |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Anti-β-Catenin-Cy3 antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the 15B8 hybridoma, produced by fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with recombinant chicken catenin. β-catenin is 92 kDa. β-catenin protein is composed of a series of protein-protein interaction motifs that allow it to function as a scaffold. The N-terminus domain contains the binding site for β-catenin as well as phosphorylation sites recognized by Glycogen synthase kinase 3 beta (GSK3β) and the C-terminus contains the transcriptional activation domain and binding site for Teashirt. |
| General description: | The gene β-catenin (CTNNB1) is mapped to human chromosome 3p21. The protein localizes to the cytoplasm and the nucleus. β-catenin is also present at the centrosomes. |
| Immunogen: | recombinant chicken β-catenin. |
| Legal Information: | Cy is distributed under license from Amersham Biosciences Limited. |
| Packaging: | 200 μL in glass insert |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |