Collagen from human placenta
SIGMA/C7774 - Bornstein and Traub Type I (Sigma Type VIII), powder
CAS Number: 9007-34-5
EC Number: 232-697-4
MDL Number: MFCD00130825
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: C7774-25MG
| biological source | human placenta |
| form | powder |
| impurities | HIV, hepatitis B and hepatitis C, none detected |
| Quality Level | 200  |
| storage temp. | 2-8°C |
| technique(s) | cell culture | mammalian: suitable |
| UniProt accession no. | P02666  |
| P02668 |
|
| P53420 |
|
| Q01955 |
|
| Q14031 |
| Application: | This product is intended to produce thin layer coatings on tissue culture plates to facilitate attachment of anchorage-dependent cells, recommended for use at 6-10 μg/cm2. It is NOT intended for production of 3-D gels. Type I collagen is often used in cell culture as an attachment substratum with myoblasts, spinal ganglia, hepatocytes, embryonic lung, heart explants, fibroblasts, endothelial cells, and islet cells have all been cultured successfully on films or gels of type I collagen. Collagen type I may also be used in research of Idiopathic pulmonary fibrosis (IPF), studies on the effect of ER stress IPF on lung fibroblasts. Collagen in acidic solution can produce three dimensional scaffolding with use in bioengineering and cell culture applications. |
| Biochem/physiol Actions: | Type I collagen is a component of skin, bone, tendon, and other fibrous connective tissues. |
| Other Notes: | All collagen molecules are composed of three polypeptide chains arranged in a triple helical conformation, with a primary structure that is mostly a repeating motif with glycine in every third position and proline or 4-hydroxyproline frequently preceding the glycine residue. Type I collagen differs from other collagens by its low lysine hydroxylation and low carbohydrate composition. |
| Other Notes: | Collagen is classified into a number of structurally and genetically distinct types. We use the nomenclature proposed by Bornstein and Traub. Be wary of confusing Sigma-type designations with recognized collagen classification types. |
| Packaging: | 5, 25 mg in glass bottle |
| Preparation Note: | Prepared by a modification of the pepsin extraction and salt fractionation method of Niyibizi, C., et al., J. Biol. Chem., 259, 14170 (1984). It is an acid soluble collagen that can be dissolved in water with acetic acid added to pH 3.0 (5 mg/mL), yielding an opalescent, colorless solution. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352202 |