Monoclonal Anti-Collagen, Type III antibody produced in mouse
SIGMA/C7805 - clone FH-7A, ascites fluid
Synonym: Anti-Collagen III antibody
MDL Number: MFCD00239753
Product Type: Chemical
Western Blotting JOURNAL CITATION: A new antifibrotic target of Ac-SDKP: inhibition of myofibroblast differentiation in rat lung with silicosis. By: Xu, H., Yang, F., et al. in PLoS One, 2012. PubMed ID: 22802960 Image collected and cropped by CiteAb from the following publication, (https://dx.plos.org/10.1371/journal.pone.0040301), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunohistochemistry Staining of frozen rat skin sections with 1:8,000 Monoclonal Anti Collagen, Type III Clone: FH-7A (Cat. No. C7805) using biotin/ExtrAvidin®-Peroxidase.
Immunohistochemistry Staining of formalin-fixed, paraffin-embedded rat skin with 1:4,000 Monoclonal Anti Collagen, Type III Clone: FH-7A (Cat. No. C7805) using biotin/ExtrAvidin®-Peroxidase.
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | FH-7A, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| isotype | IgG1 |
| mol wt | antigen 70 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | dot blot: suitable |
| immunohistochemistry (frozen sections): 1-4,000 using frozen sections of human skin | |
| indirect ELISA: suitable | |
| western blot: suitable using a denatured-reduced preparation | |
| UniProt accession no. | P02461  |
| Application: | Monoclonal anti-collagen type III antibody has been used in immunofluorescence and western blot. |
| Application: | Monoclonal Anti-Collagen, Type III antibody produced in mouse has also been used in enzyme linked immunosorbent assay (ELISA) and immunohistochemistry. |
| Biochem/physiol Actions: | Monoclonal Anti-Collagen Type III reacts specifically with native and denatured collagen type III. It does not recognize collagen types I, II, IV, V, VI, and X. |
| Biochem/physiol Actions: | This collagen is directly or indirectly involved in several genetic diseases like Ehlers-Danlos type IV disease. Monoclonal anti-collagen type III antibody can be used to determine the interstitial collagen I and III content in total protein homogenates. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Collagen is a fibrous protein present in the extracellular framework of all vertebrates. Collagen type III is a 300 kD molecule that belongs to fibrillar collagens family found mainly in skin, blood vessels, liver, placenta, tongue and thymus. This collagen is directly or indirectly involved in several genetic diseases like Ehlers-Danlos type IV disease. Monoclonal anti-collagen type III antibody can be used to determine the interstitial collagen I and III content in total protein homogenates. It is also used to study the immunolocalization of collagen type III in normal and neoplastic tissue. Mouse monoclonal anti-collagen type III antibody reacts specifically with native and denatured collagen type III but not with collagen types I, II, IV, V, VI, and X. |
| General description: | Monoclonal Anti-Collagen Type III (mouse IgG1 isotype) is derived from the FH-7A hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with human collagen type III. |
| Immunogen: | human collagen type III |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |