Creatine Phosphokinase from bovine heart
SIGMA/C7886 - Type III, salt-free, lyophilized powder, ≥30 units/mg protein
Synonym: ATP: Creatine N-Phosphotransferase; CPK; Creatine Kinase; Phosphocreatine phosphokinase
CAS Number: 9001-15-4
EC Number: 232-585-5
MDL Number: MFCD00130876
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: C7886-17.5KU
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: C7886-1KU
| biological source | bovine heart |
| description | Contains primarily isozyme MM |
| form | salt-free, lyophilized powder |
| mol wt | 80 kDa |
| Quality Level | 200  |
| specific activity | ≥30 units/mg protein |
| storage temp. | −20°C |
| type | Type III |
| Analysis Note: | Protein determined by biuret. |
| Application: | Creatine phosphokinase from bovine heart has been used to investigate whether endothelial cell growth is stimulated by ischemic hearts. Creatine phosphokinase from bovine heart has also been used to evaluate the effect of high but nontoxic dietary intake of copper and selenium on metabolism in calves. |
| Application: | Creatine Phosphokinase from bovine heart has been used:
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| Biochem/physiol Actions: | Creatine kinase plays a key role in the energy metabolism of cells with intermittently high and fluctuating energy requirements. Examples of such cells include cardiac or skeletal muscle cells and neural tissues of brain and retina. The enzyme catalyzes the reversible transfer of the phosphoryl group from phosphorylcreatine to ADP, in order to generate ATP. The molecular mass of the protein is found to be approximately 80,000 Da. It is made up of 2 subunits, each having a molecular weight of 40,000 ± 2000. The lighter subunit is present in larger amounts.CPK is released from the cellular cytosol into the systemic circulation due to disruption of cell membranes caused by hypoxia or other injuries. Furthermore, it serves as a diagnostic indicator for rhabdomyolysis and acute myocardial infarction (AMI), along with other medical disorders such as acute muscle injury, arrhythmias, and congestive cardiac failure. |
| Disclaimer: | The use of 0.1% albumin in the reaction buffer is recommended to avoid inactivation due to dilution. |
| General description: | Research area: Cell Signaling Creatine phosphokinase (CPK) is an intracellular enzyme found predominantly in skeletal muscle, brain, and myocardium. CPK consists of two polypeptide subunits, namely M (muscle type) and B (brain type). The lighter subunit is present in larger amounts. The genes encoding these subunits are on distinct chromosomes, with B on 14q32 and M on 19q13. These subunits facilitate the development of three tissue-specific isoenzymes: CPK-MB (cardiac muscle), CPK-MM (skeletal muscle), and CPK-BB (brain). |
| Other Notes: | One unit will transfer 1.0 μmole of phosphate from phosphocreatine to ADP per min at pH 7.4 at 30 °C. |
| Packaging: | 500, 1000, 3500, 17500 units in poly bottle |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥30 units/mg protein |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 2.7.3.2 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |