Anti-CD3, T Cell antibody produced in rabbit
SIGMA/C7930 - whole antiserum
Synonym: Anti-CD3epsilon; Anti-IMD18; Anti-T3E; Anti-TCRE
MDL Number: MFCD00162554
Product Type: Chemical
Immunofluorescence Human Tonsil stained with Anti-CD3, T Cell (Cat. No. C7930) at a 1:150 dilution (red) and Monoclonal Anti-Proliferating Cell Nuclear Antigen (Cat. No. P8825) at a 1:500 dilution (green). Counterstained with DAPI. From Pamela Wirth, Plastic Surgery Department, Vanderbilt University, Nashville, TN.
Western Blotting Mouse thymus extract were separated on SDS-PAGE and probed with Anti-CD3, T Cell antibody produced in Rabbit (Cat. No. C7930). The antibody was developed using 1:5,000 Anti-Rabbit IgG (whole molecule)-Alkaline Phosphatase antibody produced in Goat (Cat. No. A9919). Lanes 1. 1:200 antibody 2. Negative Control
| antibody form | whole antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| mol wt | antigen 20 kDa (bovine and swine) |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | rat, canine, mouse, human, Psammomys (sand rat), sheep, feline, pig, chicken, bovine |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200 using enzyme pre-digested, formalin-fixed, paraffin-embedded human tonsil sections |
| western blot: 1:200 using mouse thymus extract | |
| UniProt accession no. | P07766  |
| Application: | Anti-CD3, T Cell antibody produced in rabbit has been used in: • immunoblotting • immunofluorescence staining • immunohistochemistry • immunohistochemical analysis |
| Biochem/physiol Actions: | CD3 is a human cell surface glycoprotein complex involved in signal transduction following binding of antigen receptor. |
| Biochem/physiol Actions: | The antibody reacts with the proline-rich peptide sequence comprising 13 amino acids in the cytoplasmic domain of the CD3-ε chain. It detects normal, reactive and most neoplastic human T cells in routinely processed enzyme pre-digested, formalin-fixed, paraffin-embedded tissue sections. The antibody also reacts with human tissues fixed with Bouin′s solution and embedded in paraffin. Frozen sections and smears are also suitable for staining with this antibody. The antibody reacts with T cell areas in lymphoid tissues derived from various species. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | CD3 is a 20kDa glycoprotein expressed on surface of all human T lymphocytes. CD3 molecule is encoded by the gene mapped to human chromosome 11q23.3. The CD3 antigen is expressed during thymopoiesis. It is also present in the majority of T-CLL and T-lymphomas and in approximately 70% of T-ALL. |
| Immunogen: | synthetic human CD3 peptide (residues 156-168 of the CD3-ε chain) coupled to porcine thyroglobulin. Coupling was performed by a modification of the reported method. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |