Monoclonal Anti-Collagen, Type X antibody produced in mouse
SIGMA/C7974 - clone COL-10, ascites fluid
Synonym: Anti-Col10; Anti-Col10a-1
MDL Number: MFCD02686071
Product Type: Chemical
Immunohistochemistry Staining of formalin-fixed, paraffin-embedded human ostochondroma sections with 1:1,000 Monoclonal Anti-Collagen, Type X, Clone: COL-10 (Cat. No. C7974) using biotin/ExtrAvidin®-Peroxidase.
Immunofluorescence HT1080 cells were fixed and permeabilized with methanol followed by aceton. Fixed cells were stained with 1:1,000 Monoclonal Anti-Collagen, Type X, Clone: COL-10 (Cat. No. C7974). The antibody was developed using Goat Anti-Mouse IgG, Cy3™ conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | COL-10, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| isotype | IgM |
| mol wt | antigen 60 kDa (in denatured-reduced preparations) |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | deer, human, pig |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | dot blot: suitable |
| immunocytochemistry: 1:1,000 using HT 1080 human fibrosarcoma cells | |
| western blot: suitable | |
| UniProt accession no. | Q03692  |
| Application: | Monoclonal Anti-Collagen, Type X antibody produced in mouse has been used in • enzyme linked immunosorbent assay (ELISA) • dot-blot • immunoblotting and • immunohistochemistry |
| Biochem/physiol Actions: | Type X collagen is a product of hypertrophic chondrocytes. Type X collagen is non-fibrillar, but forms fine pericellular filaments in association with cartilage collagen. It interacts with matrix proteins, such as connexin V, chondrocalcein, collagen II and proteoglycans, as well as with Ca2+. It acts as a scaffold to avoid local collapse during endochondral ossification and also has a role in cartilage mineralization. Mutations in the NC1-encoding domain of the human α1(X) collagen gene, are associated with Schmid metaphysical chondroplasia. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Collagens are extracellular glycoproteins made up of three polypeptides that intermingle to form a triple helix. Type X collagen is a homotrimer of 59 kD α1(X) chains found in fetal hypertrophic cartilage in the growth zones of long bones, vertebrae and ribs, whereas in adults it is also present in thyroid cartilage. Monoclonal anti-collagen, type X antibody can be used in histological and immunohistochemical evaluation of cell cultures. It is also useful in study of specific differential tissue expression. |
| General description: | Monoclonal Anti-Collagen, Type X (mouse IgM isotype) is derived from the COL-10 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with porcine collagen type X. Collagen type X shares a similar domain structure with type VIII collagen: a central triple-helical (COL1) domain of 50 kDa is flanked by N-terminal (NC2) and C-terminal (NC1) non-triple-helical domains. |
| Immunogen: | purified pig collagen type X. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |