Cholera Toxin from Vibrio cholerae
SIGMA/C8052 - ≥90% (SDS-PAGE), lyophilized powder
Synonym: CTX; Cholera enterotoxin; Choleragen
CAS Number: 9012-63-9
MDL Number: MFCD00130776
Product Type: Chemical
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| assay | ≥90% (SDS-PAGE) |
| composition | Protein, ~5% Lowry-TCA |
| form | lyophilized powder |
| Quality Level | 300  |
| solubility | H2O: 10 mg/mL |
| storage temp. | 2-8°C |
| Application: | Cholera Toxin from Vibrio cholerae has been used as a positive control in cAMP (cyclic AMP) assay for enterotoxins. It has been added as a supplement in cell culture media of primary tumors and epithelial cells. |
| Application: | Cholera Toxin from Vibrio cholerae has been used in toxin neutralization assays to assess the cytotoxicity of cells. |
| Biochem/physiol Actions: | Toxin consisting of an A subunit (27 kDa) surrounded by five B subunits (approximately 12 kDa each), which attach the toxin to ganglioside GM1 on the cell surface. |
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| General description: | Cholera toxin is a member of the AB5-subunit family of toxins. It consists of a single A subunit responsible for ADP-ribosylation and five B subunits arranged as a pentameric ring with cell surface receptor binding and internalization functions. The A subunit catalyzes the ADP-ribosylation of the α-subunit of the stimulatory G protein (Gαs), leading to increased adenylyl cyclase activity and cAMP levels. It also ADP-ribosylates transducing and tubulin. Cholera toxin and its A subunit are commonly used to study signal transduction mechanisms due to their impact on adenylate cyclase. Additionally, cholera toxin acts as an adjuvant by stimulating B lymphocytes and T helper cell type 2 responses by inhibiting interleukin-12 production. The non-toxic B subunit (CTB) attaches to cells by binding to ganglioside GM1, making it a useful label for microglial cells in neurological research. It has also proved to be an excellent tracer for axonal transport studies using immunohistochemical methods. |
| Packaging: | Package size based on protein content. |
| Physical form: | Lyophilized powder containing Tris buffer salts, sodium chloride, sodium azide and sodium EDTA |
| Preparation Note: | When reconstituted at 1 mg/mL in water, solution will contain 0.05 M Tris buffer salts, pH 7.5, 0.2 M NaCl, 0.003 M NaN3, and 0.001 M sodium EDTA. Store reconstituted solutions in the refrigerator. |
| Symbol |  GHS06 |
| Signal word | Danger |
| Hazard statements | H300 + H310 - H332 - H412 |
| Precautionary statements | P262 - P273 - P280 - P301 + P310 - P302 + P352 + P310 - P304 + P340 + P312 |
| Hazard Codes | T |
| Risk Statements | 24/25-52/53 |
| Safety Statements | 36/37-45-61 |
| RIDADR | UN 3462 6.1 / PGII |
| WGK Germany | WGK 2 |
| Purity | ≥90% (SDS-PAGE) |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352200 |