Monoclonal Anti-Connexin-43 antibody produced in mouse
SIGMA/C8093 - clone CXN-6, ascites fluid
Synonym: Mouse monoclonal antibody
MDL Number: MFCD00803532
Product Type: Chemical
Immunohistochemistry Staining of formalin-fixed, paraffin-embedded mouse heart sections with 1:100 Monoclonal Anti-Connexin 43, Clone: CXN-6 (Cat. No. C8093) using biotin/ExtrAvidin®-Peroxidase.
Immunofluorescence C2C12 cells were fixed and permeabilized with methanol followed by acetone. Fixed cells were stained with 1:500 Monoclonal Anti-Connexin 43, Clone: CXN-6 (Cat. No. C8093). The antibody was developed using Goat Anti-Mouse IgG, ATTO488 conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.
Immunoblotting Mouse brain extract was separated on SDS-PAGE and probed with Monoclonal Anti-Connexin 43 Clone: CXN-6 (Cat. No. C8093). The antibody was developed with Alk Phos APA Mouse Fab Ads HIgG (Cat. No. A1293) and a NBT/BCIP substrate. Antibody dilution 1/16,000.
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | CXN-6, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| isotype | IgM |
| mol wt | antigen 43 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | rat, hamster, bovine, mouse, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: suitable |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable | |
| immunohistochemistry (frozen sections): suitable | |
| indirect ELISA: suitable | |
| microarray: suitable | |
| western blot: 1:8,000-1:16.000 using mouse whole brain extract | |
| UniProt accession no. | P17302  |
| Application: | Monoclonal Anti-Connexin-43 antibody produced in mouse has been used in: • fluorescent immunostaining • western blotting • immunocytochemistry • preparation of immuno-affinity columns |
| Biochem/physiol Actions: | Monoclonal Anti-Connexin-43 reacts specifically with connexin-43. Reactivity has been observed with human, bovine, hamster, rat, and mouse connexin-43. |
| Biochem/physiol Actions: | Mutations in connexin 43 (Cx43) gene result in pleiotropic phenotype of oculodentodigital dysplasia. Connexin 43 participates in the multiplication and differentiation of mature T cells. It also participates in the secretion and manufacturing of cytokines. It helps to release lactate from glycolytic pancreatic ductal adenocarcinoma cells. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-Connexin-43 (mouse IgM isotype) is derived from the CXN-6 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Connexin 43 (Cx43) is also known as GJA1 (gap junction protein alpha 1). The structure of connexin molecules includes a cytoplasmic N-terminal region, four transmembrane domains, two extracellular loops and a C-terminal cytoplasmic tail of varying length. |
| General description: | The 43 kDa connexin protein (connexin-43, Cx43) belongs to the a-type (group II) subfamily of connexin proteins. It is expressed in most tissues, even though the pattern of expression may differ in various cell types. For example, in the brain it is found in astrocytes, ependyma and leptomeninges but not in neurons, oligodendrocytes and pinealocytes; in the liver it is present in Ito cells but not hepatocytes. |
| Immunogen: | synthetic connexin-43 peptide (362-381). |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |