Chymase human
SIGMA/C8118 - recombinant, expressed in Pichia pastoris
Synonym: Mast cell protease I
CAS Number: 97501-92-3
MDL Number: MFCD00132173
Product Type: Chemical
Chymase is a cathepsin G-like, S1 serine proteinase found primarily in mast cells. It has a molecular mass of ~30 kDa, however its apparent molecular mass on SDS-PAGE is around 37 kDa.
SDS-PAGE Purity of Chymase Human (Cat. No. C8118) was assessed on SDS-PAGE.
| application(s) | diagnostic assay manufacturing |
| concentration | 125-400 μg/mL |
| form | liquid |
| mol wt | ~37 kDa by SDS-PAGE |
| Quality Level | 300  |
| recombinant | expressed in Pichia pastoris |
| shipped in | dry ice |
| specific activity | ≥40 units/mg protein |
| storage temp. | −20°C |
| UniProt accession no. | P23946  |
| Application: | Chymase has been implicated in generation of angiotensin II and cleavage of big endothelin. Studies indicate it may be involved in vascular proliferation, myocardial infarction and dermatitis. |
| Application: | Human chymase has been used in a study to assess the effects of Panax notoginseng flower extract on the TGF-β/Smad signal transduction pathway in heart remodeling. Human chymase has also been used in a study to investigate the blood glucose level and survival in streptozotocin-treated human chymase transgenic mice. |
| Biochem/physiol Actions: | The enzyme rapidly converts angiotensin I to angiotensin II. Optimum pH for the enzyme activity is between 7.5 and 9.0. Enzyme activity is inhibited by soybean trypsin inhibitor, phenylmethylsulfonyl fluoride and chymostatin. |
| General description: | Chymase is a cathepsin G-like, S1 serine proteinase found primarily in mast cells. It has a molecular mass of ~30 kDa, however its apparent molecular mass on SDS-PAGE is around 37 kDa. |
| Other Notes: | One unit hydrolyzes one micromole of N-benzoyl-L-tyrosine ethyl ester (BTEE) per minute at pH 7.8 and 25 °C. The assay buffer used to determine the enzyme activity contains 27 mM Tris-HCl, pH 7.8, with 150 mM NaCl and 0.43 mM BTEE. |
| Physical form: | Supplied as a solution in 20 mM Tris, 0.8 M NaCl and 25% glycerol, pH 7.6 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥40 units/mg protein |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 3.4.21.39 ( BRENDA | IUBMB ) |
| UNSPSC | 12352204 |