Collagenase from Clostridium histolyticum

SIGMA/C8176 - Sigma Blend Type L, ≤1.0 FALGPA units/mg solid

Synonym: Clostridiopeptidase A

CAS Number: 9001-12-1
EC Number: 232-582-9
MDL Number: MFCD00130830
Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-C8176-5G	5 g	$5850.00 1/EA	Add To Favorites

Sigma collagenases are fully characterized by enzymatic activity. Many are specifically tested for suitability for cell dissociation and cell viability for hepatocytes, adipocytes, and pancreatic cells.

Unit Definition One collagen digestion unit (CDU) liberates peptides from collagen from bovine achilles tendon equivalent in ninhydrin color to 1.0 μmole of leucine in 5 hours at pH 7.4 at 37 °C in the presence of calcium ions.

Properties
Descriptions
Safety Info.
Basic Data

caseinase activity	≥40 units/mg solid
form	powder
mol wt	68-130 kDa
Quality Level	200
specific activity	≤1.0 FALGPA units/mg solid
storage temp.	−20°C

Application:	Collagenase from Clostridium histolyticum, or Clostridiopeptidase A, has been used in a study to assess the synthesis and in vitro evaluation of macromolecular antitumour derivatives based on phenylenediamine mustard. Clostridiopeptidase A has also been used in a study to investigate the enzymatic debridement of burn wounds with collagenase.
Biochem/physiol Actions:	Collagenase is activated by four gram atom calcium per mole enzyme. It is inhibited by ethylene glycol-bis(beta-aminoethyl ether) - N, N, N′,N′-tetraacetic acid, beta-mercaptoethanol, glutathione, thioglycolic acid and 8-hydroxyquinoline.
Biochem/physiol Actions:	Effective release of cells from tissue requires the action of collagenase enzymes and the neutral protease. Collagenase is activated by four gram atom calcium (Ca²⁺) per mole enzyme. The culture filtrate is thought to contain at least 7 different proteases ranging in molecular weight from 68-130 kDa. The pH optimum is 6.3-8.8. The enzyme is typically used to digest the connective components in tissue samples to liberate individual cells. Ethylene glycol-bis(β-aminoethyl ether)-N,N,N′,N′-tetraacetic acid (EGTA)4; β-mercaptoethanol; glutathione, reduced; thioglycolic acid, sodium; and 2,2′-dipyridyl; 8-hydroxyquinoline are known to inhibit the enzyme activity.
Other Notes:	One collagen digestion unit (CDU) liberates peptides from collagen from bovine achilles tendon equivalent in ninhydrin color to 1.0 μmole of leucine in 5 hours at pH 7.4 at 37 °C in the presence of calcium ions. One FALGPA hydrolysis unit hydrolyzes 1.0 μmole of furylacryloyl-Leu-Gly-Pro-Ala per min at 25°C. One Neutral Protease unit hydrolyzes casein to produce color equivalent to 1.0 μmole of tyrosine per 5 hr at pH 7.5 at 37°C. One Clostripain Unit hydrolyzes 1.0 μmole of BAEE per min at pH 7.6 at 25°C in the presence of DTT.

Symbol	GHS08
Signal word	Danger
Hazard statements	H334
Precautionary statements	P261 - P284 - P501
Hazard Codes	Xn
Risk Statements	36/37/38-42
Safety Statements	22-24-26-36/37
RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

activity	caseinase activity: ≥40 units/mg solid; specific activity: ≤1.0 FALGPA units/mg solid
Storage Temp.	−20°C
Enzyme Commission (EC) Number	3.4.24.3 ( BRENDA \| IUBMB )
UNSPSC	12352204

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